| 三元能量诱变妥布拉霉素突变株生物合成研究 |
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| 引用本文： | 顾觉奋,王鲁燕,陈正尧.三元能量诱变妥布拉霉素突变株生物合成研究[J].人天科学研究,1996(5). |
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| 作者姓名： | 顾觉奋 王鲁燕 陈正尧 |
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| 作者单位： | 中国药科大学抗生素研究室，中国药科大学抗生素研究室，中国元极学研究会科技应用委员会 南京，210009，南京，210009，鄂州，436000 |
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| 基金项目： | 江苏省自然科学基金，中国元极学研究会科研基金 |
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| 摘 要： | 通过正交试验的方法考察了NH_4Cl、磷酸盐、(NH_4)_2SO_4、MgSO_4、ZnSO_4、CoCl_2、NoCl、FeSO_4、MnSO_4等无机盐对三元能量处理后的妥布拉霉素(TOB)诱变菌株发酵水平及主组份含量的影响.实验结果表明,以NHCI的影响最为显著,K_3HPO_4次之,NaCI最小,用正交方法研究了几种无机盐之间的交互作用,以Zn~(2+)与Mn~(2+)之间的交互影响最为显著,选择出了最适有机氮源,获得了三元能量诱变株的最佳发酵培养基,从而使发酵单位提高了41.06％,主组份含量由40.53％提高到53.40％.
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| 关 键 词： | 三元能量 诱变株 正交试验 生物合成 妥布拉霉素 |
A Study of Biosynthesis of Three-Yuan Mutagenic Tobramycin Mutant Strain |
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| Authors: | Gu Juefen female prof Pharmaceutical University of China Nanjing G PRC |
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| Institution: | Gu Juefen female,prof. , Pharmaceutical University of China,Nanjing,21G009,PRC. |
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| Abstract: | t An examination is conducted of the influence of such inorganic salts as NH4C1, phosphate, (NH4)2SO4, MgSO4, ZnSO4, CoCl2,NaCl, FeSO4, and MnSO4 upon the fermentativity and the principal constituent content of the tobramycin-mutagenic fungi, using the method of orthogonal test, whose results show that NH4C1 exercises greatest influence. K2HPO4 less, NaCl least; using the orthogonal method, a study is made of the interaction of kinds of inorganic salts, showing that Zn2+ and Mn2+ exert greatest mutual influence; and the most proper organic nitrogen source is selectd, and the best fermentative substrata for Three-Yuan mutagenic fungi is prepared, so that the fermentation units increase by 41. 06% , and the content of the principal constituent increase from 40. 53% to 53. 40%. |
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| Keywords: | Three-Yuan energy mutagenic fungi orthogonal test biosynthesis tobramycin |
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