Evaluation of antioxidant profile and activity of amalaki (Emblica officinalis), spirulina and wheat grass |
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Authors: | Vasudha Shukla Manish Vashistha Som Nath Singh |
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Institution: | (1) Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi, 110054, India;(2) Nutrition Division, Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi, 110054, India |
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Abstract: | Aqueous and alcoholic extracts of amalki (Emblica officinalis), spirulina and wheatgrass were prepared and analyzed for antioxidant
vitamin content (vitamin C and E), total phenolic compounds. Antioxidant status, reducing power and effect on glutathione
S-transferase (GST) activity were evaluated in vitro. Vitamin C content of crude amalaki powder was found to be 5.38 mg/g,
while very less amount 0.22 mg/g was detected in wheat grass. Amalki was rich in vitamin E like activity, total phenolic content,
reducing power and antioxidant activity. Total antioxidant activity of aqueous extract of amalki, spirulina and wheat grass
at 1mg/ml concentration were 7.78, 1.33 and 0.278 mmol/l respectively. At similar concentrations the total antioxidant activity
of alcoholic extract of amalaki, spirulina and wheat grass was 6.67, 1.73 and 0.380 mmol/l respectively. Amalki was also found
to be rich source of phenolic compounds (241mg/g gallic acid equivalent). Alcoholic extract of wheat grass showed 50 % inhibition
in FeCl2- ascorbic acid induced lipid peroxidation of rat liver homogenates in vitro. Both aqueous and alcoholic extracts of amalaki
inhibited activity of rat liver glutathione S-transferase (GST) in vitro in dose dependant manner. Since GST acts as powerful
drug metabolizing enzyme its inhibition by amalaki offers possibility of its use for lowering therapeutic dose of herbal preparations.
The aqueous extracts of both amalki and spirulina also showed protection against t-BOOH induced cytotoxicity and production
of ROS in cultured C6 glial cells. |
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Keywords: | Amalki Emblica officinalis Spirulina Wheat grass Nutraceutical Vitamin C |
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