Interleukin-1 receptor antagonist gene polymorphism and obesity: A pilot study from north India |
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| Authors: | P K Manchanda H K Bid B R Achyut B Mittal N Srivastava R D Mittal |
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| Institution: | (1) Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, 226014 Lucknow;(2) Department of Physiology, King George Medical University, 226014 Lucknow;(3) Department of Urology, Sanjay Gandhi Postgraduate Institute-of Medical Sciences, 226014 Lucknow, India |
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| Abstract: | The main adverse consequences of excess bodyweight are cardiovascular disease, type II diabetes, and several cancers, IL-1Ra
serum concentration has been reported earlier to increase in human obesity and it is therefore assumed that the polymorphism
of IL-1Ra may influence cytokine production. We designed this study to investigate whether the IL-1Ra polymorphism was associated
with obesity. A total number of 103 individuals; 19 lean (BMI<25 Kg/m2), 51 overweight (BMI 25–29.9 Kg/m2) and 33 obese (BMI≥30.0 Kg/m2) were enrolled in this study. Genotyping was performed using a polymerase chain reaction PCR amplification of the intron-2
fragment harboring a variable number of tandem repeat (VNTR) nucleotide sequences 86 pb of tandem repeat. The PCR products
were separated on 2% agarose gel. Statistical analysis was performed using SPSS software (version 11.5). We found no significant
difference in genotype and allele frequencies between the three groups; lean vs. overweight and lean vs. obese (p=0.323; 0.202;
0.123 and 0.068 resp). However, an increased risk for obesity had a propensity to be higher in those having genotype II/II.
This genotype has been reported to be a ‘high producer’ of IL-1Ra. Although no statistically significant relationship between
IL-1Ra polymorphism and BMI was observed, however, a trend towards an increase of allele*II in overweight and obese group was observed. This may suggest that IL-1Ra appears to be induced by inflammatory stimuli
as well as obesity-associated factors. This is relatively a pilot study: but nevertheless, may assist in identifying the pathophysiological
cause for obesity. |
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| Keywords: | IL-1 Ra Polymorphism VNTR Single nucleotide polymorphisms (SNP) Obesity |
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