| Capillary method for measuring near-infrared spectra of microlitre volume liquids |
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| 作者姓名： | YUAN Bo MURAYAMA Koichi |
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| 作者单位： | Department of |
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| 摘 要： | INTRODUCTION The bands of near-infrared (NIR) spectra have low absorptivities, which allow in situ analysis of chemical and biological materials with no or little sample preparation. Therefore, as one of the most important nondestructive analytical techniques, NIR spectroscopy is being extensively applied in the fields of agriculture, industry, biotechnology and medicine (Siesler et al., 2002; Scarff et al., 2006; Ward et al., 2006). Recently, with instrumental developments such as Fou…
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| 关 键 词： | 毛细管 近红外光谱 微升 光学 数值分析 |
| 收稿时间： | 2006-03-17 |
| 修稿时间： | 2006-08-11 |
Capillary method for measuring near-infrared spectra of microlitre volume liquids |
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| YUAN Bo MURAYAMA Koichi.Capillary method for measuring near-infrared spectra of microlitre volume liquids[J].Journal of Zhejiang University Science,2007,8(2):171-175. |
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| Authors: | Yuan Bo Murayama Koichi |
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| Institution: | (1) Department of Information Science and Optoelectronic Engineering, State Key Laboratory of Modern Optical Instrumentation, CNERC for Optical Instrument, Zhejiang University, Hangzhou, 310027, China;(2) Department of Biochemistry and Biophysics, Gifu University School of Medicine, Gifu 500-8705, Japan |
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| Abstract: | The present study theoretically explored the feasibility of the capillary method for measuring near-infrared (NIR) spectra of liquid or solution samples with microlitre volume, which was proposed in our previous studies. Lambert-Beer absorb- ance rule was applied to establish a model for the integral absorbance of capillary, which was then implemented in numerical analyses of the effects of capillary on various spectral features and dynamic range of absorption measurement. The theoretical speculations indicated that the capillary method might be used in NIR spectroscopy, which was further supported by the empirical data collected from our experiments by comparison between capillary NIR spectra of several organic solvents and cuvette cell NIR spectra. |
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| Keywords: | Near-infrared (NIR) spectroscopy Capillary Cuvette cell Numerical analysis |
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