Improvement of ethanol production from birch and spruce pretreated with 1-H-3-methylmorpholinium chloride |
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| Institution: | 1. Department of Chemical Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran;2. Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 412 96 Göteborg, Sweden;3. Department of Chemical Engineering, Faculty of Engineering, University of Isfahan, Isfahan 81746-73441, Iran;4. Industrial Biotechnology Group, Research Institute for Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan 84156-83111, Iran;5. Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran;6. Department of Chemistry, College of Sciences, Shiraz University, Shiraz 71467-13565, Iran;7. Department of Chemistry, Technical Chemistry, Chemical-Biological Centre, Umeå University, 90187 Umeå, Sweden;8. Industrial Chemistry & Reaction Engineering, Johan Gadolin Process Chemistry Centre, ÅboAkademi University, 20500 Åbo-Turku, Finland |
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| Abstract: | BackgroundPretreatment is the critically important step for the production of ethanol from lignocelluloses. In this study, hardwood birch (Betula pendula) and softwood spruce (Norway spruce) woods were pretreated with a newly synthesized morpholinium ionic liquid, 1-H-3-methylmorpholinium chloride (HMMorph]Cl]), followed by enzymatic hydrolysis and fermentation to ethanol.ResultsHMMorph]Cl] was synthesized using inexpensive raw materials, i.e., hydrochloric acid and N-methyl morpholine, following a simple process. The influence of pretreatment time (2, 3, 5, and 8 h) and temperature (120 and 140°C) in terms of hydrolysis efficiency was investigated. Glucose yields from enzymatic hydrolysis were improved from 13.7% to 45.7% and 12.9% to 51.8% after pretreatment of birch and spruce woods, respectively, under optimum pretreatment conditions (i.e., at 140°C for 3 h) as compared to those from pristine woods. Moreover, the yields of ethanol production from birch and spruce were increased to 34.8% and 44.2%, respectively, while the yields were negligible for untreated woods.ConclusionsThis study demonstrated the ability of HMMorph]Cl] as an inexpensive agent to pretreat both softwood and hardwood.How to cite: Mohammadi M, Shafiei M, Karimi K. Improvement of ethanol production from birch and spruce pretreated with 1‐H‐3‐methylmorpholinium chloride. Electron J Biotechnol 2019;41. https://doi.org/10.1016/j.ejbt.2019.07.004. |
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