| 保幼激素Ⅲ对蛹虫草液体发酵生长代谢的调控作用 |
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| 引用本文： | 施明珠,李有贵,钟石,李增智,计东风.保幼激素Ⅲ对蛹虫草液体发酵生长代谢的调控作用[J].科技通报,2009,25(1). |
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| 作者姓名： | 施明珠 李有贵 钟石 李增智 计东风 |
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| 作者单位： | [1]安徽农业大学微生物防治重点实验室,合肥230036 [2]浙江省农业科学院蚕桑研究所,杭州310021 |
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| 基金项目： | 浙江省重大国际科技合作项目资助(2004C14009) |
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| 摘 要： | 通过对菌丝体生物量、胞外多糖产量、胞内多糖及虫草素进行测量分析，研究了不同水平的保幼激素Ⅲ对蛹虫草液体发酵培养的调控作用。在培养的早期阶段（1d），保幼激素Ⅲ对蛹虫草菌丝体生长有明显的抑制作用，但其最终产量与对照组相比仍有一定程度的增加．其中高剂量组增加了14．3％。各处理组胞外多糖产量在培养3d后即达到对照组第7天的水平．而胞内多糖含量及虫草素含量在培养ld后迅速增加，与同期对照组相比分别达到140％和362％。各处理组在整个培养过程中的生长代谢节奏与对照组有差异性。胞内多糖及虫草素产量分析表明．保幼激素Ⅲ在12mg／L水平下使得虫草素产量增加了31．2％。但对胞内多糖产量无促进作用。结果表明．保幼激素Ⅲ改变了蛹虫草菌丝体生长代谢节奏，对处于特定菌龄期（5d）的蛹虫草生长代谢具有调控作用。
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| 关 键 词： | 保幼激素Ⅲ 调控作用 液体发酵 蛹虫草 |
Regulatory Effect of Juvenile Hormone III on the Growth and Metabolism of Cordyceps militaris by Liquid Fermentation |
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| SHI Mingzhu,LI Yougui,ZHONG Shi,LI Zengzhi,JI Dongfeng.Regulatory Effect of Juvenile Hormone III on the Growth and Metabolism of Cordyceps militaris by Liquid Fermentation[J].Bulletin of Science and Technology,2009,25(1). |
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| Authors: | SHI Mingzhu LI Yougui ZHONG Shi LI Zengzhi JI Dongfeng |
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| Institution: | 1.Key Laboratory of Microbial Control of Anhui province;Hefei 230036;China;2.Zhejiang Academy of Agricultural Science;Hangzhou 310021;China |
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| Abstract: | Regulatory effect of different concentrations of Juvenile hormone III on the mycelial growth,extracellular polysaccharide(EPS),intracellular polysaccharide(IPS)and cordycepin were studied in this paper.During the early stage of fermentation(about 1day),Juvenile hormone III had an inhibitory effect on the mycelial growth,but the final outputs increased to a certain extent compared with the control group.Thereinto,high level group increased by a factor of 14.3%.The maximal EPS yields of the test groups were o... |
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| Keywords: | juvenile hormone III regulatory effect liquid fermentation Cordyceps militaris |
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