| 不同肥料处理下茶园土壤细菌和古菌群落的时间变化研究 |
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| 作者姓名： | Hua WANG ;Shao-hui YANG ;Jing-ping YANG ;Ya-min LV ;Xing ZHAO ;Ji-liang PANG |
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| 作者单位： | [1]Institute of Environmental Protection, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; [2]College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China |
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| 基金项目： | Project supported by the Research Fund for the Doctoral Program of Higher Education of China （No. 20130101110127） and the Project of Zhejiang Key Scientific and Technological Innovation Team （No. 2010R50039）, China |
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| 摘 要： | 研究目的：研究化学肥料和有机肥处理条件下，茶园酸性土壤细菌和古菌群落结构，以及氮素转化相关功能酶基因丰度的时间变化规律。
创新要点：研究肥料、土壤温度及土壤含水量对茶园酸性土壤细菌和古菌群落结构，以及氮素转化相关功能酶基因丰度的影响。
研究方法：应用末端限制性片段长度多态性（T-RFLP）技术分析茶园酸性土壤中细菌和古菌群落结构随时间的变化规律，应用荧光定量聚合酶链式反应（PCR）技术，研究茶园酸性土壤细菌、古菌、硝化作用功能酶基因（细菌和古菌amod基因）和细菌反硝化作用功能酶基因（narG、nirK、nirS和nosZ基因）丰度的时间变化规律。
重要结论：茶园土壤细菌和古菌群落结构受到肥料的影响，并随着取样时间有显著的变化。细菌、古菌和古菌的amoA基因的丰度在7月份最小，而细菌的amoA基因和反硝化作用功能酶基因（除nirK基因）的丰度在9月份最小。有机肥处理增加了细菌、古菌和氮素转化相关功能酶基因的丰度，但化学肥料的施用对菌群及功能酶基因丰度的影响较小。土壤温度显著影响了土壤细菌和古菌的群落结构。土壤含水量与细菌反硝化作用功能酶基因有显著的相关性。土壤有机碳含量与细菌、古菌及功能酶基因丰度之间有显著的相关性。
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| 关 键 词： | 茶园 时间变化 肥料 细菌和古菌群落 氮素转化相关功能酶基因 |
| 收稿时间： | 2014 Apr 22 |
Temporal changes in soil bacterial and archaeal communities with different fertilizers in tea orchards |
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| Hua WANG,;Shao-hui YANG,;Jing-ping YANG,;Ya-min LV,;Xing ZHAO,;Ji-liang PANG.Temporal changes in soil bacterial and archaeal communities with different fertilizers in tea orchards[J].Journal of Zhejiang University Science,2014,15(11):953-965. |
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| Authors: | Hua Wang Shao-hui Yang Jing-ping Yang Ya-min Lv Xing Zhao Ji-liang Pang |
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| Institution: | 1. Institute of Environmental Protection, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China
2. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China
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| Abstract: | It is important to understand the effects of temporal changes in microbial communities in the acidic soils of tea orchards with different fertilizers. A field experiment involving organic fertilizer （OF）, chemical fertilizer （CF）, and unfertilized control （CK） treatments was arranged to analyze the temporal changes in the bacterial and archaeal communities at bimonthly intervals based on the 16S ribosomal RNA （rRNA） gene using terminal restriction fragment length polymorphism （T-RFLP） profiling. The abundances of total bacteria, total archaea, and selected functional genes （bacterial and archaeal amoA, bacterial narG, nirK, nirS, and nosZ） were determined by quantitative poly- merase chain reaction （qPCR）. The results indicate that the structures of bacterial and archaeal communities varied significantly with time and fertilization based on changes in the relative abundance of dominant T-RFs. The abundancy of the detected genes changed with time. The total bacteria, total archaea, and archaeal amoA were less abundant in July. The bacterial amoA and denitrifying genes were less abundant in September, except the nirK gene. The OF treatment increased the abundance of the observed genes, while the CF treatment had little influence on them. The soil temperature significantly affected the bacterial and archaeal community structures. The soil moisture was signif- icantly correlated with the abundance of denitrifying genes. Of the soil chemical properties, soil organic carbon was the most important factor and was significantly correlated with the abundance of the detected genes, except the nirK gene. Overall, this study demonstrated the effects of both temporal alteration and organic fertilizer on the structures of mi- crobial communities and the abundance of genes involved in the nitrogen cycle. |
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| Keywords: | Bacterial and archaeal communities Fertilizer Soil Temporal changes Tea orchard Functional genes |
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