| Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue （MIL-C） by Streptoverticillium rimofaciens ZJU 5119 |
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| 作者姓名： | Ye D Xu ZN Cen PL |
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| 作者单位： | Dan YE（Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China） ;
Zhi-nan XU（Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China） ;
Pei-lin CEN（Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China） ; |
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| 基金项目： | 科技部科研项目,浙江省科技厅资助项目 |
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| 摘 要： |
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| 关 键 词： | 胞核嘧啶 核苷抗生素 产量 介质最优化 |
| 收稿时间： | 10 July 2007 |
| 修稿时间： | 30 September 2007 |
Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by <Emphasis Type="Italic">Streptoverticillium rimofaciens</Emphasis> ZJU 5119 |
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| Ye D,Xu ZN,Cen PL.Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119[J].Journal of Zhejiang University Science,2008,9(1):77-84. |
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| Authors: | Dan YE Zhi-nan Xu Pei-lin CEN |
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| Institution: | Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China |
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| Abstract: | Cytosine-substituted mildiomycin analogue (MIL-C) was produced effectively by supplementing cytosine into the culture of Streptoverticillium rimofaciens. In order to improve the yield of MIL-C, statistically-based experimental designs were applied to optimize the fermentation medium for S. rimofaciens ZJU 5119. Fifteen culture conditions were examined for their significances on MIL-C production using Plackett-Burrnan design. The Plackett-Burman design and one-variable-at-a-time design indicated that glucose and rice meal as the complex carbon sources, and peanut cake meal and NH4NO3 as the complex nitrogen sources were beneficial for MIL-C production in S. rimofaciens ZJU 5119. The results of further central composition design (CCD)showed that the optimal concentration of glucose, rice meal and peanut cake meal were 18.7 g/L, 64.8 g/L and 65.1 g/L,respectively. By using this optimal fermentation medium, the MIL-C concentration was increased up to 1336.5 mg/L, an approximate 3.8-fold improvement over the previous concentration (350.0 mg/L) with un-optimized medium. This work will be very helpful to the large-scale production of MIL-C in the future. |
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| Keywords: | Cytosine-substituted mildiomycin analogue (MIL-C) Plackett-Burman design Response surface methodology Streptoverticillium rimofaciens Nucleoside antibiotic |
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