| Pathogenicity of bacterium, Xenorhabdus nematophila isolated from entomopathogenic nematode （Steinernema carpocapsae） and its secretion against Galleria mellonella larvae |
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| 作者姓名： | Mahar AN Munir M Elawad S Gowen SR Hague NG |
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| 作者单位： | Department of Agriculture,University of Reading,Reading RG6 6AT,UK,School of Plant Sciences,University of Reading,Reading RG6 6AS,UK,Department of Agriculture,University of Reading,Reading RG6 6AT,UK,Department of Agriculture,University of Reading,Reading RG6 6AT,UK,Department of Agriculture,University of Reading,Reading RG6 6AT,UK |
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| 摘 要： | INTRODUCTION Entomopathogenic nematodes of the generaSteinernema and Heterorhabditis have been used tocontrol a wide range of agriculturally important insectpests (Kaya and Gaugler, 1993). The mechanism bywhich these nematodes are able to infect and repro-duce in the insect host involves a mutual relationshipbetween the nematode and the symbiotic bacteria,Xenorhabdus spp. and Photorhabdus spp. (Akhurstand Dunphy, 1993; Forst and Nealson, 1996). Poinarand Thomas (1966) established tha…
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| 关 键 词： | 生物学控制 细菌共生 病原菌 线虫病 |
Pathogenicity of bacterium, Xenorhabdus nematophila isolated from entomopathogenic nematode (Steinernema carpocapsae) and its secretion against Galleria mellonella larvae |
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| Mahar AN,Munir M,Elawad S,Gowen SR,Hague NG.Pathogenicity of bacterium, Xenorhabdus nematophila isolated from entomopathogenic nematode (Steinernema carpocapsae) and its secretion against Galleria mellonella larvae[J].Journal of Zhejiang University Science,2005,6(6):457-463. |
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| Authors: | Mahar Ali Nawaz Munir Muhammad Elawad Sami Gowen Simon Richard Hague Nigel Graham Meckenzi |
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| Institution: | Department of Agriculture, University of Reading, Reading RG6 6AT, UK. |
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| Abstract: | The entomopathogenic bacterium, Xenorhabdus nematophila was isolated from the hemolymph of Galleria mellonella infected with Steinernema carpocapsae. The bacterial cells and its metabolic secretions have been found lethal to the Galleria larvae. Toxic secretion in broth caused 95% mortality within 4 d of application whereas the bacterial cells caused 93%mortality after 6 d. When filter and sand substrates were compared, the later one was observed as appropriate. Similarly, bacterial cells and secretion in broth were more effective at 14% moisture and 25 ℃ temperature treatments. Maximum insect mortality (100%) was observed when bacterial concentration of4×106 cells/ml was used. Similarly, maximum bacterial cells in broth (95%)were penetrated into the insect body within 2 h of their application. However, when stored bacterial toxic secretion was applied to the insects its efficacy declined. On the other hand, when the same toxic secretion was dried and then dissolved either in broth or water was proved to be effective. The present study showed that the bacterium, X. nematophila or its toxic secretion can be used as an important component of integrated pest management against Galleria. |
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| Keywords: | Biological control Bacterial symbionts Entomopathogenic nematodes Xenorhabdus nematophila Galleria mellonella |
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