Human cytomegalovirus induces alteration of β-actin mRNA and microfilaments in human embryo fibroblast cells |
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| Authors: | Lin Mao-fang Wei Guo-qing Huang He Cai Zhen |
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| Institution: | (1) Department of Hematology, First Affiliated Hospital, College of Medical Science, Zhejiang University, 310003 Hangzhou, China |
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| Abstract: | Objective: To investigate the infection of human embryo fibroblast cell line HF cells by CMV as well as the effects of CMV
on β-actin mRNA and microfilaments. Methods: HF cells shape was observed after the infection of CMV. RT-PCR assay was used
to detect the mRNA expression of CMV immediate early (IE) gene, β-actin and GAPDH genes of HF cells infected by CMV. CMV particles
and cell microfilaments were detected with electron microscope. Results: Shape of HF cell changed after the infection by CMV.
HF cells infected by CMV could express IE mRNA and the expression of β-actin mRNA decreased in a time-and titer-dependent
manner compared with the uninfected HF cells whose expression of GAPDH mRNA did not change much. CMV particles were found
with electron microscope in the cells. Microfilaments were ruptured and shortened after the infection of CMV. Conclusion:
CMV can not only infect human embryo fibroblast cells line HF cells and replicate in the cells, but can also affect the expression
of β-actin mRNA and the microfilaments.
Project (No. 001103058) supported by the Key Program of Science and Technology Bureau of Zhejiang Province, China |
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| Keywords: | Cytomegalovirus Human embryo fibroblast cells Infection β -actin Microfilament |
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