| 大蹼铃蟾的线粒体基因组D—loop区序列分析 |
| |
| 引用本文： | 张雁,韩毛振,罗学才,王晶晶.大蹼铃蟾的线粒体基因组D—loop区序列分析[J].巢湖师专学报,2013(6):71-75. |
| |
| 作者姓名： | 张雁 韩毛振 罗学才 王晶晶 |
| |
| 作者单位： | [1]合肥师范学院生命科学系,安徽合肥230061 [2]安徽大学生命科学学院,安徽合肥230601 |
| |
| 基金项目： | 安徽省高校省级自然科学基金项目（项目编号：KJ2011Z314）;合肥师范学院自然科学研究项目（项目编号：2011KJ11） |
| |
| 摘 要： | 脊椎动物的线粒体DNA（mtDNA）是一段长度约为15—22Kb的环形片段，具有相对的保守性。D-loop区是线粒体基因组中的非编码区域的一部分，具有控制和调节的功能。本文采用LA—PCR和生物信息学的方法，研究了大蹼铃蟾的线粒体基因组D-loop区序列，其D-loop区具有三个保守序列（CSB-1，CSB-2 and CSB-3），在5、和3、端还有独特的串联重复序列。通过分析总结其串联重复序列的特征和规律，有助于研究蛙科动物线粒体基因组进化机制。
|
| 关 键 词： | 大蹼铃蟾 线粒体基因组 D—loop 串联重复序列 |
SEQUENCE ANALYSIS OF D-LOOP REGION OF MITOCHONDRIAL GENOME OF BOMBINA MAXIMA |
| |
| Authors: | ZHANG Yan HAN Mao-zhen LUO Xue-cai WANG Jing-jing |
| |
| Institution: | 1 ( 1 Departmont of Life Science, Hefei Normal University, Hefei Anhui 230061 ) (2 School of Life Science,Anhui University, Hefei Anhui 230601) |
| |
| Abstract: | Mitochondrial DNA （mtDNA） is a circular genome in vertebrate,whose length is about 15-22Kb,and it has a relatively conservative. D-loop region with control and regulation functions, which is a part of non-coding in the mitochondrial genome. To study mitochondrial genome of Bombina Maxima, the sequences of D-loop region are analyzed by using LA-PCR, and bioinformatics. Three conserved sequences （CSB-1, CSB-2 and CSB-3） are found in D-loop region of the mitochondrial genome of Bombina Maxima, and tandem repeated sequences are found both in the 5＇ ends and 3＇ ends. Analyzing and summa- rizing the regular pattern of tandem repeated sequences of Mitochondrial DNA in Bombina Maxima has a great important signif- icance for studying the evolutionary mechanism of mitochondrial genome in Ranidae. |
| |
| Keywords: | Bombina Maxima mitochondrial DNA （mtDNA） D-loop tandem repeated sequences |
| 本文献已被 维普 等数据库收录！ |
