| 无患子丛生芽诱导及植株再生研究 |
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| 引用本文： | 林杰,冯永涛,陈民良,刘希华,邢建宏.无患子丛生芽诱导及植株再生研究[J].三明高等专科学校学报,2013(4):83-87. |
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| 作者姓名： | 林杰 冯永涛 陈民良 刘希华 邢建宏 |
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| 作者单位： | 三明学院资源与化工学院,福建三明365004 |
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| 基金项目： | 国家大学生创新创业训练计划项目（201211311012）;福建省质量工程建设项目（ZL1001/JT（sj）） |
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| 摘 要： | 以无患子叶片为外植体，通过正交试验，研究无患子叶片愈伤组织诱导、愈伤组织诱导丛生芽、芽苗生根以及植株再生的最佳培养基组成。并对试验数据进行极差分析和方差分析。结果表明：诱导出叶片愈伤组织的最佳培养基是MS＋0．5mg·L^-1 TDZ＋0．4mg·L^-12,4-D＋6．0mg·L～AgN03，诱导率为86．4％。诱导丛生芽的最佳培养基是MS＋0．5mg·L^-1NAB．＋1．0mg·L^-1TDZ，分化率为42．5％。诱导生根的最佳培养基是1／3MS＋0．5mg·L^-16-BA＋0．5mg·L^-12,4-D，生根率为45％。
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| 关 键 词： | 无患子 愈伤组织 丛生芽 植株再生 |
Study on Induction of Multiple Shoot and Plantlet Regeneration of Sapindus mukorossi Gaerth |
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| LIN Jie,FENG Yong-tao,CHEN Min-liang,LIU Xi-hua,XING Jian-hong.Study on Induction of Multiple Shoot and Plantlet Regeneration of Sapindus mukorossi Gaerth[J].Journal of Sanming College,2013(4):83-87. |
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| Authors: | LIN Jie FENG Yong-tao CHEN Min-liang LIU Xi-hua XING Jian-hong |
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| Institution: | (School of Resources and Chemical Engineering, Sanming University, Sanming 365004, Chian) |
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| Abstract: | The best mediums of the callus induction, multiple shoot induction, rooting and plant regeneration were studied by orthogonal design with using the leaves ofSapindus mukorossi Gaerth. as materials in this paper, and the experimental data were analyzed by range analysis and variance analysis. The results showed that the best culture medium for inducing callus from the leaves of Sap indus mukorossi Gaerth. is MS supplemented with 0.5 mg-L＂~ TDZ ＋ 0.4 mg. L-~ 2,4-D ＋ 6 rag- L＂~ AgNO3, with an induction rate of 86.4%. The best culture medium for inducing multiple shoot is MS supplemented with 0.5 mg.L^-1 NAA ＋ 1.0 mg. L^-1 TDZ, and induction rate of 42.5%. The best culture medium for inducing rooting is 1/3 MS ＋ 0.5 mg-L^-1 6-BA ＋ 0.5 mg-L^-1 2,4-D, and induction rate is 45%. |
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| Keywords: | Sapindus mukorossi Gaerth orthogonal design callus multiple shoot planflet regeneration |
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