Assessment of genetic diversity by simple sequence repeat markers among forty elite varieties in the germplasm for malting barley breeding |
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| Authors: | Jun-mei Wang Jian-ming Yang Jing-huan Zhu Qiao-jun Jia and Yue-zhi Tao |
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| Institution: | (1) Research Institute for Bioresources, Okayama University, 2-20-1 Chuo, Kurashiki Okayama Prefecture, 710-0046, Japan;(2) Present address: National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba Ibaraki, 305-8602, Japan; |
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| Abstract: | The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat (SSR)
genotyping data. The amplified fragments from SSR primers were highly polymorphic in the barley accessions investigated. A
total of 85 alleles were detected at 35 SSR loci, and allelic variations existed at 29 SSR loci. The allele number per locus
ranged from 1 to 5 with an average of 2.4 alleles per locus detected from the 40 barley accessions. A cluster analysis based
on the genetic similarity coefficients was conducted and the 40 varieties were classified into two groups. Seven malting barley
varieties from China fell into the same subgroup. It was found that the genetic diversity within the Chinese malting barley
varieties was narrower than that in other barley germplasm sources, suggesting the importance and feasibility of introducing
elite genotypes from different origins for malting barley breeding in China. |
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| Keywords: | Barley (Hordeum vulgare L ) Genetic similarity Simple sequence repeat (SSR) marker Cluster analysis Genetic diversity |
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