| 外周血单个核细胞感染乙型肝炎病毒在母婴垂直传播中的作用 |
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| 引用本文： | 苏雪松,马洁.外周血单个核细胞感染乙型肝炎病毒在母婴垂直传播中的作用[J].河北北方学院学报(医学版),2004,21(2):3-5. |
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| 作者姓名： | 苏雪松 马洁 |
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| 作者单位： | 1. 河北省秦皇岛市第三医院妇产科,066000
2. 河北省秦皇岛市妇幼保健医院,066000 |
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| 摘 要： | 目的:探讨HBsAg、HBeAg阳性孕妇外周血单个核细胞(PBMC)内乙型肝炎病毒(HBV)DNA感染状况及其在宫内母婴垂直传播中的作用.方法:对HBsAg/HBeAg双阳性共67对孕妇及其新生儿静脉血分离和提纯PBMC后,经抽提、纯化后的DNA进入PCR扩增反应,引物为HBV C区基因序列.结果:67名HBsAg及HBeAg双阳性的孕妇中有35例(52.2%)PBMC中HBV DNA阳性,25例孕妇在血清及PBMC中均发现HBV DNA.67名新生儿有22例感染HBV DNA,感染率32.8%,其中血清HBV DNA阳性者10例,PBMC HBVDNA阳性者19例,二者均阳性者7例.结论:母亲PBMC内HBV DNA阳性可能导致新生儿PBMC中HBV DNA阳性,PBMC内的HBV DNA可能是HBV母婴垂直传播的一条重要途径,同时,HBsAg及HBeAg阳性母亲若血清HBV DNA为阳性就极大增加了其新生儿感染HBV的危险性.
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| 关 键 词： | 肝炎病毒 乙型 疾病传播 垂直 白细胞 单核 聚合酶链反应 |
| 文章编号： | 1005-8796(2004)02-03-03 |
Role of Peripheral Blood Mononuclear Cell Infected Hepatitis B Virus in the Intrauterine Transmission |
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| Su Xuesong,Ma Jie Third Hospital of Qinhuangdao,Qinhuangdao.Role of Peripheral Blood Mononuclear Cell Infected Hepatitis B Virus in the Intrauterine Transmission[J].Journal of Hebei North University:Medical Edition,2004,21(2):3-5. |
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| Authors: | Su Xuesong Ma Jie Third Hospital of Qinhuangdao Qinhuangdao |
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| Institution: | Su Xuesong,Ma Jie Third Hospital of Qinhuangdao,Qinhuangdao,066000 |
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| Abstract: | Objective:The purpose of this study was to determine the HBV infection rate of PBMC in pregnant women with both postive HBsAg and HBeAg and their newborn and to evaluate the role of infected PBMC in the intrauterine. Methods: 67 mothers- infants pairs participated in the study, PBMC were isolated from the same blood sample. The DNA was extracted and purified, then DNA samples were set for the PCR procedure using the primers specific for the c gene sequence. Results:35(52.2%) of the 67 positive HBsAg and HBeAg pregnant women had been detected PBMC HBV DNA and serum HBV DNA, respectively. 25 of whom were all detected HBV specific DNA in PBMC and plasma samples.22(32.8%)of 67 newborns infected HBV, including 10 newborns with serum HBV DNA and 19 newborns with PBMC HBV DNA, 7 newborns were all detected HBV DNA in PBMC and plasma sample. Conclusion:That infection with HBV in maternal PBMC is responsible for perinatal newborn's PBMC HBV infection and it may be an important route of HBV vertical transmission. Infants whose mothers had sera positive for both HBsAg and HBeAg and HBV DNA had extraordinarily high risk of hepatitis B virus infection. |
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| Keywords: | hepatitis B vertical transinission moncnuclear cell |
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