Targeted cell detection based on microchannel gating |
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| Authors: | Mehdi Javanmard AmirAli H Talasaz Mohsen Nemat-Gorgani Fabian Pease Mostafa Ronaghi and Ronald W Davis |
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| Institution: | Electrical Engineering Department, Stanford University, Stanford, California 94305, USA and Stanford Genome Technology Center, Palo Alto, California 94304, USA;Stanford Genome Technology Center, Stanford Genome Technology Center, Palo Alto, California 94304, USA;Electrical Engineering Department, Stanford University, Stanford, California 94305, USA;Stanford Genome Technology Center, Stanford Genome Technology Center, Palo Alto, California 94304, USA |
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| Abstract: | Currently, microbiological techniques such as culture enrichment and various plating techniques are used for detection of pathogens. These expensive and time consuming methods can take several days. Described below is the design, fabrication, and testing of a rapid and inexpensive sensor, involving the use of microelectrodes in a microchannel, which can be used to detect single bacterial cells electrically (label-free format) in real time. As a proof of principle, we have successfully demonstrated real-time detection of target yeast cells by measuring instantaneous changes in ionic impedance. We have also demonstrated the selectivity of our sensors in responding to target cells while remaining irresponsive to nontarget cells. Using this technique, it can be possible to multiplex an array of these sensors onto a chip and probe a complex mixture for various types of bacterial cells. |
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