Development and validation of a low cost blood filtration element separating plasma from undiluted whole blood |
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| Authors: | Homsy Alexandra van der Wal Peter D Doll Werner Schaller Roland Korsatko Stefan Ratzer Maria Ellmerer Martin Pieber Thomas R Nicol Andreas de Rooij Nico F |
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| Institution: | 1Ecole Polytechnique Fédérale de Lausanne, SAMLAB, Neuchâtel, Switzerland;2Department of Internal Medicine, Division of Endocrinology and Metabolism, Medical University of Graz, Graz, Austria;3Joanneum Research Forschungsgesellschaft mbH HEALTH—Institute of Biomedicine and Health Sciences, Graz, Austria;4Bayer Technology Services GmbH, Leverkusen, Germany |
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| Abstract: | Clinical point of care testing often needs plasma instead of whole blood. As centrifugation is labor intensive and not always accessible, filtration is a more appropriate separation technique. The complexity of whole blood is such that there is still no commercially available filtration system capable of separating small sample volumes (10-100?μl) at the point of care. The microfluidics research in blood filtration is very active but to date nobody has validated a low cost device that simultaneously filtrates small samples of whole blood and reproducibly recovers clinically relevant biomarkers, and all this in a limited amount of time with undiluted raw samples. In this paper, we show first that plasma filtration from undiluted whole blood is feasible and reproducible in a low-cost microfluidic device. This novel microfluidic blood filtration element (BFE) extracts 12?μl of plasma from 100?μl of whole blood in less than 10?min. Then, we demonstrate that our device is valid for clinical studies by measuring the adsorption of interleukins through our system. This adsorption is reproducible for interleukins IL6, IL8, and IL10 but not for TNFα. Hence, our BFE is valid for clinical diagnostics with simple calibration prior to performing any measurement. |
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