结合LAMP-CRISPR/Cas12b与热敏感尿嘧啶DNA糖苷酶实现可消除残留污染的副溶血弧菌快速可视化检测(英文) |
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引用本文: | 吴芳,卢辰,胡文灏,郭馨,陈佳玥,罗志丹.结合LAMP-CRISPR/Cas12b与热敏感尿嘧啶DNA糖苷酶实现可消除残留污染的副溶血弧菌快速可视化检测(英文)[J].浙江大学学报(B卷英文版),2023(8):749-763. |
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作者姓名: | 吴芳 卢辰 胡文灏 郭馨 陈佳玥 罗志丹 |
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作者单位: | 1. 江苏海洋大学,江苏省海洋生物资源与环境重点实验室,江苏省海洋药物活性分子筛选重点实验室;2. 江苏海洋大学,江苏省海洋生物产业技术协同创新中心 |
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摘 要: | <正>Vibrio parahaemolyticus is a major pathogen frequently found in seafood. Rapid and accurate detection of this pathogen is important for the control of bacterial foodborne diseases and to ensure food sa-fety. In this study, we established a one-pot system that combines uracil-DNA glycosylase(UDG), loopmediated isothermal amplification(LAMP), and clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 12b(Cas12b) for detecting V. parahaemolyticus in sea...
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关 键 词: | 尿嘧啶糖苷酶 环介导等温扩增 CRISPR/Cas12b 副溶血弧菌 一锅法检测关 |
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