| 柑橘和瓯柑橘皮类黄酮清除羟基自由基的效果 |
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| 引用本文： | 张静.柑橘和瓯柑橘皮类黄酮清除羟基自由基的效果[J].南平师专学报,2011,30(5):36-39. |
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| 作者姓名： | 张静 |
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| 作者单位： | 武夷学院茶学与生物系,福建武夷山,354300 |
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| 基金项目： | 福建省教育厅《欧柑橘类黄酮提取及药理作用研究》课题内容(项目编号:JA08247) |
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| 摘 要： | 以柑橘皮和瓯柑橘皮类黄酮为研究对象，利用Fenton反应产生的羟自由基与染料试剂溴甲酚紫发生氧化反应后使其吸光值降低，间接测定它们对羟自由基（·OH）的清除效果。结果：lmg／mL柑橘皮类黄酮，在0．20-0．80ml的使用剂量范围内，对·OH清除效果呈正比增加趋势（P〈0．01），超过O．80ml后开始略有降低；lmg／mL欧柑橘皮类黄酮，在0．10-1．0ml的使用剂量范围内，对·OH清除效果也呈正比增加趋势（P〈0．01），超过1．0mL后开始下降。同等剂量下瓯柑橘皮类黄酮对·OH清除效果显著高于柑橘皮类黄酮。
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| 关 键 词： | 溴甲酚紫 羟基自由基 柑橘皮 瓯柑橘皮 类黄酮 硫脲 |
Scavenging Effect on Hydroxyl Radical by Flavonoids from Citrus Suavissima Peel and Orange Peel |
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| ZHANG Jing.Scavenging Effect on Hydroxyl Radical by Flavonoids from Citrus Suavissima Peel and Orange Peel[J].Journal of Nanping Teachers College,2011,30(5):36-39. |
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| Authors: | ZHANG Jing |
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| Institution: | ZHANG Jing (Tea Science& Biology Department of Wuyi University,Wuyishan,Fujian 354300 ) |
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| Abstract: | Flavonoids extracted from orange peel and citrus suavissima peel was applied as the object in this study.A method was developed for determination of the hydroxyl radical in Fenton reaction. Bromocresol purple reacted with · OH, then the absorbency of the solution declined. It can be used to determine the ·OH indirectly due to the change of absorbency. The results showed that lmg/mL flavonoids from orange peel increased from 0.2mL to 0.8mL, lmg/mL flavonoids citrus suavissima peel from increased from 0.1mL to 1.0mL on the scavenging effect of ·OH is proportional to the increased （ P 〈 0.01）, and are respectively in 0.8mL and 1.0mL on the removal rate reaches a maximum, and then began to decline. The scavenging effect of ·OH from citrus suavissima peel was significantly higher than that from orange peel in the same dose. |
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| Keywords: | brom ocresol purple OH peel of orange peel of citrus suavissima flavonoids thiourea |
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