| 一株以原油为碳源的Pseudomonas sp.菌化学趋向性研究 |
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| 作者姓名： | 景贵成 郭尚平 俞理 |
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| 作者单位： | 1. 中国科学院渗流流体力学研究所, 廊坊 065007;
2. 中国石油勘探开发研究院, 北京 100083 |
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| 基金项目： | 中国石油股份公司重点科技攻关项目(0301211)资助 |
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| 摘 要： | 从某油田产出水中分离得到一株以原油为碳源的Pseudomonassp.菌(假单孢菌),其化学趋向性运动能力中等,趋向性机理在本文中得以详细描述.在可视化模型实验中可看到微生物向油水界面处的有序运动,而在有效的营养物浓度梯度范围内,化学趋向性运移速度与距油水界面的距离无关,受温度的影响很大.并且在微观仿真模型实验中,可观察到细菌在孔隙壁和油、水三相交界处聚集,并讨论了该现象的形成原因及其对微生物采油的有利作用.
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| 关 键 词： | 微生物 化学趋向性 油水界面 聚集 |
| 收稿时间： | 2004-06-08 |
| 修稿时间： | 2004-09-17 |
Studies on the Chemotaxis of a Bacterium Pseudomonas sp. Using Crude Oil as Carbon Source |
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| Authors: | JING Gui-Cheng GUO Shang-Ping YU Li |
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| Institution: | 1. Institute of Porous Flow and Fluid Mechanics, Chinese Academy of Sciences, La ngfang 065007, China;
2. Research Institute of Petroleum Exploration and Development, PetroChina, Beijing 100083, China |
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| Abstract: | A bacterium Pseudomonas sp. using the crude oil as the carbon source was separated from the production water in an oilfield. Its chemotaxis migration ability was really medium, and its chemotaxis mechanism was particularly described in this paper. In visualized experiments, the ordered motion of microorganism to the oil water interface could be watched, and within the scope of the effect ive nutriment concentration grads, the chemotaxis mot ion velocity was not related with the distance from the interface at all and it was much influenced by the temperature. Meanwhile, in experiments using emulational micro-models, it could be observed that bacteria had become enriched at the orifice wall, oil and water three-phrase boundary, and the forming reason and the beneficial effect on MEOR were also discussed. |
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| Keywords: | microorganism chemotaxis oil water interface enrichment |
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