Production of recombinant enveloped structural proteins from the Chinese WSSV isolate |
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| Authors: | Rajeev Kumar Jha Zi-rong Xu |
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| Institution: | (1) Genetic Engineering Laboratory, Feed Science Institute, Animal Science College, Zhejiang University, 310029 Hangzhou, Peoples Republic of China |
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| Abstract: | The white spot syndrome virus (WSSV) is one of the deadly pathogens of penaeid shrimps and other crustaceans. The WSSV virion
consists of an enveloped rod-shaped nucleocapsid enclosing a large circular double stranded DNA genome of 305 Kb with 181
open reading frames. The two major structural genes, VP19 and VP28 were amplified from the genomic DNA of Chinese isolate
of WSSV and cloned in pUCm-T vector and sub cloned in pET-30a (+) vector. The expressions of genes inE. coli (BL21) were confirmed by SDS-PAGE analysis. The clones were sequenced, submitted to the gene bank and the Xiang Shan strain
of WSSV were compared with the previous reported sequence of WSSV of various regions which revealed that VP19 and VP28 gene
sequences had certain differences from the sequences of similar genes of the isolate already reported. The recombinant proteins
expressed, purified and characterized. |
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| Keywords: | Shrimp White spot syndrome Virus VP19 VP28 |
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