| 基于RT-HPLC的古代mtDNA序列差异性分析 |
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| 引用本文： | 张金,张虎勤,郭晓萍.基于RT-HPLC的古代mtDNA序列差异性分析[J].绵阳师范学院学报,2007,26(5):59-63,85. |
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| 作者姓名： | 张金 张虎勤 郭晓萍 |
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| 作者单位： | 1. 绵阳师范学院分子生物学与生物制药重点实验室,四川绵阳,621000
2. 西安交通大学生物医学信息工程教育部重点实验室,陕西西安,710049 |
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| 摘 要： | 线粒体DNA具有突变速度快、重组率低、严格母系遗传、拷贝数多等特点,近年来成为分子考古研究中的一个热点。为了研究古墓群中的血亲关系,可以从古墓葬遗留骨骸、牙齿中提取和扩增mtDNA片断进行分析。通过设计2对部分重叠的引物,扩增线粒体16055-16218区域目标片段,进行反相高效液相色谱分析,确认四个样品对应片断在反相柱上的保留时间上存在差异,以此推断扩增片段在序列上可能存在差异。为利用mtDNA分析人类母系血缘关系奠定了基础。
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| 关 键 词： | RT-HPLC mtDNA 古DNA 分子考古 |
| 文章编号： | 1672-612x（2007）05-0059-05 |
| 修稿时间： | 2007-03-30 |
An Analysis of the Diversity of Ancient mtDNA Sequences by RT-HPLC |
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| ZHANG Jin,ZHANG Hu-qing,GUO Xiao-ping.An Analysis of the Diversity of Ancient mtDNA Sequences by RT-HPLC[J].Journal of Mianyang Normal University,2007,26(5):59-63,85. |
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| Authors: | ZHANG Jin ZHANG Hu-qing GUO Xiao-ping |
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| Abstract: | Mitochondrion DNA(mtDNA) is marked with quick mutation,low recombination,strict maternal inheritance,multicopy and becomes one of the hot areas of molecule archeology in recent years.We extract mtDNA segments from the human remains in ancient graves,design two overlapping primers to amplify the area from 16055 to 16218bp in mtDNA and analyze the sequence diversity by RT-HPLC.As a result of different reserved time,we suggest that the four segments amplified from the four samples differ in the nucleic acid sequences. |
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| Keywords: | RT-HPLC mtDNA ancient DNA molecule archeology |
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