Effect of Certain Antibiotics Against Filarial Parasite <Emphasis Type="Italic">Brugia malayi</Emphasis> In Vitro: Possible Role of Oxidative Stress |
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| Authors: | Rachna Sabharwal Mahajan Anandharaman Veerpathran Gajalakshmi Dakshinamoorthy Richa Dwarkaprasad Sharma Kalyan Goswami Maryada Venkatarami Reddy |
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| Institution: | (1) Department of Biochemistry and JB Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, Maharashtra, 442102, India; |
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| Abstract: | WHO-Tropical Disease Research scheme highlighted the need for development of new anti-filarial drugs. Certain antibiotics
have recently been found effective against Wolbachia, co-existing symbiotically with filarial parasites. Inflammatory response
entails oxidative mechanism to educe direct anti-microbial effect. In the present study microfilariae were maintained in vitro
in medium supplemented with varying concentrations of tetracycline, doxycycline (20–100 μg/ml) or ciprofloxacin (50–250 μg/ml)
separately to find out any involvement of oxidative mechanism in the anti-filarial effect of these antibiotics. Loss of motility
of the microfilariae was measured after 48 h and correlated with the levels of MDA, nitric oxide and protein-carbonylation.
Significant loss of microfilarial motility was recorded with increasing concentration of tetracycline and doxycycline but
with ciprofloxacin the effect was not marked. Agents with high antifilarial activity revealed significant association with
oxidative parameters in a dose dependent manner. The result suggests that oxidative effect might be exploited to design novel
antifilarial drug candidate. |
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| Keywords: | Antibiotics B malayi Oxidative stress Drug design |
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