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三种柚叶悬浮培养愈伤组织黄酮类有效成分的比较研究
引用本文:周梦春,苏智先,胡进耀,齐刚,曾艳,刘美.三种柚叶悬浮培养愈伤组织黄酮类有效成分的比较研究[J].绵阳师范学院学报,2009,28(2).
作者姓名:周梦春  苏智先  胡进耀  齐刚  曾艳  刘美
作者单位:1. 西华师范大学环境科学与生物多样性保护省级重点试验室,四川南充,637002;绵阳师范学院,四川绵阳,621000
2. 绵阳师范学院,四川绵阳,621000
3. 西华师范大学环境科学与生物多样性保护省级重点试验室,四川南充,637002
基金项目:四川省教育厅项目,绵阳市科技攻关项目 
摘    要:用MS+2,4-D1.0 mg/L+6%蔗糖培养基诱导三种柚叶片愈伤组织,悬浮培养法扩大培养.进行有效成分分析.结果表明沙田柚含有较高黄酮物质、柚皮苷和野漆树苷,结溪蜜柚次之,江津柚含量最低,约为沙田柚的50-60%.柚苷元在三个品种愈伤组织中含量相似.该结果为柚类进一步遗传转化和改良提供了基础数据.

关 键 词:柚子  愈伤组织  柚皮苷  黄酮

A Comparative Study of the Effective Components of flavonoids from Suspension-cultured Callus Among Three Citrus grandis Cultivars
ZHOU Meng-chun,SU Zhi-xian,HU Jin-yao,QI Gang,ZENG Yan,LIU Mei.A Comparative Study of the Effective Components of flavonoids from Suspension-cultured Callus Among Three Citrus grandis Cultivars[J].Journal of Mianyang Normal University,2009,28(2).
Authors:ZHOU Meng-chun  SU Zhi-xian  HU Jin-yao  QI Gang  ZENG Yan  LIU Mei
Institution:1.Key Laboratory of Environment Science and Biodiversity Conservation;Xihua Normal University;Nanchong;Sichuan 637002;2.Mianyang Normal University;Mianyang;Sichuan 621000
Abstract:Leaf calli of three Citrus grandis cultivars are cultured with MS + 2,4-D 1.0 mg/L + 6% sucrose.Then the calli are amplified by way of suspension culture to analyze the effective components.The results show that: The Shatian-Yu cultivar has the highest level of flavonoids,naringin and rhoifolin,while the Guanxi-Miyu cultivar has a little lower level,and the Jiangjin-Yu cultivar has the lowest level(about 50-60% of the Guanxi-Miyu cultivar's).The naringenin contents in the leaf calli from the three cultivars...
Keywords:Citrus grandis L    callus  naringin  flavonoid  
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