Optimization of Bacillus subtilis cell growth effecting jiean-peptide production in fed batch fermentation using central composite design |
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| Institution: | 1. Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, PR China;2. Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, PR China;3. University of Chinese Academy of Sciences, Beijing 100049, PR China |
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| Abstract: | BackgroundOptimization of nutrient feeding was developed to improve the growth of Bacillus subtilis in fed batch fermentation to increase the production of jiean-peptide (JAA). A central composite design (CCD) was used to obtain a model describing the relationship between glucose, total nitrogen, and the maximum cell dry weight in the culture broth with fed batch fermentation in a 5 L fermentor.ResultsThe results were analyzed using response surface methodology (RSM), and the optimized values of glucose and total nitrogen concentration were 30.70 g/L and 1.68 g/L in the culture, respectively. The highest cell dry weight was improved to 77.50 g/L in fed batch fermentation, which is 280% higher than the batch fermentation concentration (20.37 g/L). This led to a 44% increase of JAA production in fed batch fermentation as compared to the production of batch fermentation.ConclusionThe results of this work improve the present production of JAA and may be adopted for other objective products' production. |
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