Bioconversion of mixed free fatty acids to poly-3-hydroxyalkanoates by Pseudomonas putida BET001 and modeling of its fermentation in shake flasks |
| |
| Institution: | Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia |
| |
| Abstract: | BackgroundThe paper reports on the utilization of palm kernel oil (PKO) as a low cost renewable substrate for medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) production by Pseudomonas putida BET001. Investigation on the effects of selected key variables on growth, mixed free fatty acids consumption and mcl-PHA production by the bacterial culture in the shaken flask system were carried out along with its kinetic modeling.ResultsThe biomass production, fatty acids consumption and mcl-PHA production were found favorable when the strain was cultured in mineral medium at pH 6–7, 28°C, aeration surface-to-volume ratio of 0.4 × 106 m- 1, 250 rpm agitation rate for 48 h. Mcl-PHA production by this strain showed mixed growth and non-growth associated components as described by Luedeking–Piret kinetic model.ConclusionThe findings of this study provided add to the literature on key variables in for achieving good microbial growth and mcl-PHA production in shake flasks culture. In addition, suitable kinetic model to describe cultivation in this system was also presented. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
