| 中国特有濒危裸子植物白豆杉RAPD反应体系的优化 |
| |
| 作者单位： | 广东化工制药职业技术学院，中山大学生命科学学院，中山大学生命科学学院 广东广州510520中山大学生命科学学院，广东广州510275，广东广州510275，广东广州510275 |
| |
| 摘 要： | 采用改进的CTAB法提取中国特有濒危裸子植物白豆杉(Pseudotaxus chienii)嫩叶总DNA,进行RAPD分析.分别研究了模板DNA、引物、dNTPs、Mg2 和TaqDNA聚合酶用量对RAPDPCR扩增反应结果的影响.筛选并建立了适合于白豆杉RAPD扩增的反应体系:总体积25μL,其中10×PCR缓冲液(500mmol/L KCl,100mmol/L Tris-HCl,1.0%Triton X-100,20mmol/L MgCl2)2.0μL,引物(1.5μmol/L)0.5μL,dNTPs(10mmol/L)0.4μL,模板1μL(35ng),Taq酶0.8μL(0.5U),水20.3μL.
|
| 关 键 词： | 白豆杉 RAPD 反应体系 优化 |
Optimal Choice for Reaction System in RAPD Analysis of Pseudotaxus Chienii (Taxaceae) |
| |
| Authors: | OUYANG Pu-yue SU Ying-juan YI Su |
| |
| Institution: | OUYANG Pu-yue1,2,SU Ying-juan2,YI Su2 |
| |
| Abstract: | The DNA templates of Pseudotaxus chienii(Taxaceae)extracted from young leaf were amplified by RAPD.Effects of the content of DNA temlates,primers,dNTP,Mg2 and Taq DNA polymerase on experimental results were tested and the optimal reaction system of RAPD for Pseudotaxus Chienii(Taxaceae)was determined as follows:35 ng DNA template,2.0 mmol/L Mg2 ,0.018 umol/L primer,250 umol/L dNTPs,0.5 u Taq DNA polymerase in total 25 uL reaction volume. |
| |
| Keywords: | Pseudotaxus chienii(Cheng)Cheng RAPD reaction system optimal choice |
| 本文献已被 CNKI 等数据库收录！ |
|
