| 产油脂酵母菌株的筛选及发酵条件的初步研究 |
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| 引用本文： | 李艾,;郝玉芹.产油脂酵母菌株的筛选及发酵条件的初步研究[J].唐山学院学报,2014(3):87-90. |
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| 作者姓名： | 李艾 ;郝玉芹 |
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| 作者单位： | [1]唐山学院环境与化学工程系,河北唐山063000; [2]唐山学院基础教学部,河北唐山063000 |
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| 摘 要： | 对14份土样和7株实验室保藏菌株经初筛、摇瓶复筛,得到1株油脂产量较高的酵母菌,其油脂含量为7.34%。对影响该菌体产油脂的条件进行了单因素和正交实验,得出摇瓶发酵培养的最佳产油脂条件:发酵周期为4d,酵母膏和硫酸铵混合物为氮源,接种量为12%。在该条件下测得的菌体生物量为12.211g/L,油脂产量为1.524g/L,油脂含量为12.48%。
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| 关 键 词： | 产油脂酵母 油脂含量 发酵条件 |
A Study of Screening of Lipid Yeast and Fermentation Conditions |
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| Institution: | LI Aia, HAO Yu-qinb (a. Department of Environmental and Chemical Engineering; b. Department of Foundational Teaching, Tangshan College, Tangshan 063000, China) |
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| Abstract: | After preliminary screening and shake flask screening of the 14 soil samples and 7 strains preserved in the laboratory,the authors of this paper obtained a rich oil-yielding yeast whose fat content is 7.34%.The single factor and orthogonal experiments on the microbial lipid production conditions indicate the optimum conditions of producing oil in shaking flask culture. The fermentation period is 4 days,and when the source of yeast extract and the mixture of am-monium sulfate is nitrogen,the inoculation is 1 2%.Under these conditions the measured biomass is 12.211 g/L,oil output is1.524 g/L,and oil content is 12.48%. |
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| Keywords: | lipid yeast lipid content fermentation conditions |
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