| 运动疲劳小鼠皮层-纹状体突触可塑性受损的机制研究 |
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| 引用本文： | 马婧,陈慧敏,张凌韬,刘晓莉,乔德才.运动疲劳小鼠皮层-纹状体突触可塑性受损的机制研究[J].中国体育科技,2022(1). |
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| 作者姓名： | 马婧 陈慧敏 张凌韬 刘晓莉 乔德才 |
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| 作者单位： | 国家体育总局体育科学研究所;北京体育大学;清华大学;北京师范大学 |
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| 基金项目： | 国家体育总局体育科学研究所基本科研业务费资助项目(基本22-11);国家自然科学基金青年科学基金项目(31701038)。 |
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| 摘 要： | 目的:前期研究发现,运动疲劳后小鼠皮层-纹状体通路的突触可塑性受损。皮层-纹状体突触可塑性受谷氨酸(glutamate,Glu)能系统和多巴胺(dopamine,DA)能系统的双重调控,因此旨在进一步围绕Glu能系统和DA能系统揭示小鼠运动疲劳后皮层-纹状体通路突触可塑性受损的分子机制。方法:成年C57BL/6雄性小鼠,随机分为对照组(Control)和运动疲劳组(EF)。利用电动跑台,使小鼠进行连续7天的力竭跑台运动,创建运动疲劳的小鼠模型;采用高效液相色谱分析技术,检测两组小鼠纹状体脑区Glu和DA的浓度;采用免疫印迹技术,检测小鼠纹状体膜蛋白中AMPA受体、代谢型谷氨酸1型受体(metabotropic glutamate receptor 1,mGluR1)、多巴胺1型受体(dopamine 1 receptor,D1R)和多巴胺2型受体(dopamine 2 receptor,D2R)的表达含量。结果:1)与Control小鼠相比,EF小鼠纹状体Glu的浓度升高(P<0.05);2)EF小鼠纹状体AMPA受体GluR1亚基和GluR2亚基的表达含量与Control小鼠相比均无差异(P>0.05);3)EF小鼠纹状体mGluR1的表达含量显著降低(P<0.01);4)EF小鼠纹状体DA的浓度降低(P<0.01);5)EF小鼠纹状体D1R和D2R的表达含量均显著降低(P<0.001)。结论:小鼠运动疲劳后纹状体Glu的浓度升高,mGluR1的表达下调;DA的浓度降低,D1R和D2R的表达下调。纹状体Glu能系统和DA能系统的双重异常可能是小鼠运动疲劳后皮层-纹状体突触可塑性受损的分子机制之一。
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| 关 键 词： | 运动疲劳 纹状体 突触可塑性 Glu能系统 DA能系统 |
Mechanism of the Impaired Corticostriatal Synaptic Plasticity in Exercise-Induced Fatigue Mice |
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| MAJing,CHEN Huimin,ZHANG Lingtao,LIU Xiaoli,QIAO Decai.Mechanism of the Impaired Corticostriatal Synaptic Plasticity in Exercise-Induced Fatigue Mice[J].China Sport Science and Technology,2022(1). |
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| Authors: | MAJing CHEN Huimin ZHANG Lingtao LIU Xiaoli QIAO Decai |
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| Institution: | (China Institute of Sport Science,Beijing 100061,China;Beijing Sport University,Beijing 100091,China;Tsinghua University,Beijing 100084,China;Beijing Normal University,Beijing 100875,China) |
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| Abstract: | Objective:Previous study had found that the corticostriatal synaptic plasticity was impaired after exercise-induced fatigue(EF).Corticostriatal synaptic plasticity is dually regulated by the glutamate(Glu)system and the dopamine(DA)system,therefore,this study aims to further reveal the molecular mechanism of impaired corticostriatal synaptic plasticity after EF,which focusing on the Glu system and DA system.Methods:Adult C57 BL/6 male mice were randomly divided into control mice and EF mice.EF mice were forced to run on an electric treadmill until fatigue daily for seven consecutive days;high performance liquid chromatography(HPLC)was used to examine the concentration of Glu and DA in the striatum of two group;Western Blot was used to detect the expressions of AMPA receptor,metabotropic glutamate receptor 1(mGluR1),dopamine 1 receptor(D1R)and dopamine 2 receptor(D2 R)in striatal membrane proteins.Results:1)Compared with control mice,the concentration of striatal Glu was increased in EF mice(P<0.05);2)the expression of striatal GluR1 and GluR2 subunits of AMPA receptor in EF mice were not significantly different from those in control mice(P>0.05);3)the expression of striatal mGluR1 was decreased in EF mice(P<0.01);4)the concentration of striatal DA was decreased in EF mice(P<0.01);5)the expression of D1 R and D2R were both decreased in the striatum of EF mice(P<0.001).Conclusions:After EF,the concentration of Glu was increased,m GluR1 expression was down-regulated;the concentration of DA was decreased,D1 R and D2 R expression were both down-regulated in the striatum.The dual abnormalities of Glu and DA systems may be the molecular mechanisms of impaired corticostriatal synaptic plasticity after EF. |
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| Keywords: | exercise-induced fatigue striatum synaptic plasticity Glu system DA system |
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