| 几种不同动物骨骼肌肌球蛋白重链(MHC)亚型及成肌调节因子(MRFs)基因表达的比较 |
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| 引用本文： | 潘同斌,钱福鸿,王晓雪,王雅一.几种不同动物骨骼肌肌球蛋白重链(MHC)亚型及成肌调节因子(MRFs)基因表达的比较[J].北京体育大学学报,2011,34(2):52-54. |
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| 作者姓名： | 潘同斌 钱福鸿 王晓雪 王雅一 |
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| 作者单位： | 扬州大学体育学院; |
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| 基金项目： | 江苏省高校自然科学基金课题(07KJD180242) |
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| 摘 要： | 目的:比较测定几种不同动物骨骼肌肌球蛋白重链(MHC)亚型及成肌调节因子(MRFs)基因表达,引物均采用大鼠基因号设计,旨在比较观察其它动物与大鼠基因表达的交叉及相似度。方法:选用大鼠、家兔、牛与人的股外侧肌样本各6个,迅速用TRIZOL试剂匀浆固定,采用实时荧光-PCR法,测定骨骼肌MHCI,IIa,IIx,IIb四种亚型及成肌调节因子(MRFs)的mRNA基因表达。结果:未经内参较正的测定值,人、牛、兔的肌球蛋白重链(MHC)亚型及成肌调节因子(MRFs)基因表达均明显低于大鼠(P<0.01),提示各物种的同种基因存在差异性,其引物特异性较强;但经过内参校正后的测定值,人的MHC各亚型基因表达与大鼠含量较接近,有一定的交叉和基因相似性;而牛的含量虽可检出,但仍明显低于大鼠含量(P<0.01),表明其基因相似性较低;兔与大鼠基因未表现出相似性。提示,各物种同种基因的表达既存在差异性,又有一定的交叉和相似性。其蛋白水平的比较测定有待进一步研究。
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| 关 键 词： | 股骼肌 基因表达 肌球蛋白重链 成肌调节因子 |
The Comparison of the Gene Expression of Myosin Heavy Chain (MHC) Isoforms and Myogenic Regulation Factors (MRFs) in Skeletal Muscle among Different Animals |
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| PAN Tong-bin,QIAN Fu-hong,WANG Xiao-xue,WANG Ya-yi.The Comparison of the Gene Expression of Myosin Heavy Chain (MHC) Isoforms and Myogenic Regulation Factors (MRFs) in Skeletal Muscle among Different Animals[J].Journal of Beijing Sport University,2011,34(2):52-54. |
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| Authors: | PAN Tong-bin QIAN Fu-hong WANG Xiao-xue WANG Ya-yi |
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| Institution: | PAN Tong-bin,QIAN Fu-hong,WANG Xiao-xue,WANG Ya-yi(Physical Education College of Yangzhou University,Yangzhou 225002,Jiangsu China) |
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| Abstract: | Objective: To compare the similarity of the gene expression of myosin heavy chain(MHC) isoforms and myogenic regulation factors(MRFs) in skeletal muscle among different animals by the primer of rat.Method: 24 samples of vastus lateralis respectively from rats,rabbits,bulls and human were fixed and homogenated with TRIZOL.Then the gene expression of MHC isoforms and MRFs in skeletal muscle among different animals was measured by realtime PCR method.Results:When not being calibrated by internal reference,the ... |
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| Keywords: | gene expression comparison myosin heavy chain(MHC) isoforms myogenic regulation factors(MRFs) |
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