| RAPD在蔬菜种子纯度鉴定中应用的反应条件优化 |
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| 引用本文： | 李成伟,李卓杰.RAPD在蔬菜种子纯度鉴定中应用的反应条件优化[J].商丘师范学院学报,2000,16(6):80-83. |
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| 作者姓名： | 李成伟 李卓杰 |
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| 作者单位： | [1]商丘师范学院生物系,河南商丘 [2]中山大学生命科学学院,广东广义 |
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| 基金项目： | 广州市科委重点攻关项目!(96 -Z- 25) |
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| 摘 要： | 随着分子生物学的发展，ＲＡＰＤ（随扩增多态性ＤＮＡ）在越来越多的领域得到应用，但在蔬菜纯度鉴定方面，国内外报道较少，为了更好地将ＲＡＰＤ方法应用于蔬菜种子纯度鉴定，就反应条件进行了探索，以几种蔬菜种子为材料，使用进口ＰＣＲ仪在，对几种蔬菜种子进行ＲＡＰＤ分子过程中，从ＤＮＡ提取方法，模板ＤＮＡ量，Ｍｇ＾２＋浓度，Ｔａｑ－ＤＮＡ聚合酶的量，ｄＮＴＰｓ浓度，随机引物浓度及反应缓冲液的量等几个方面进行研
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| 关 键 词： | RAPD 蔬菜 种子纯度 反应条件优化 |
The protocol optimum of RAPD used on the testing of vegetable seed purity |
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| LI Cheng wei ,LI Zhuo jie ,CHEN Run zheng ,ZHANG Wen hai.The protocol optimum of RAPD used on the testing of vegetable seed purity[J].Journal of Shangqiu Teachers College,2000,16(6):80-83. |
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| Authors: | LI Cheng wei LI Zhuo jie CHEN Run zheng ZHANG Wen hai |
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| Institution: | LI Cheng wei 1,LI Zhuo jie 2,CHEN Run zheng 2,ZHANG Wen hai 3 |
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| Abstract: | With the development of molecular biology,RAPD (Random amplified polymorphic DNA)was widely used in more and more biological fields,but there were fewer reports of using RAPD in the testing of vegetable sees purity in nation and abroad.In order to use RAPD in testing the vegetable seed purity measurement more freely,this paper studied the protocol optimum of RAPD.With the material of several vegetable seeds,the DNA extraction method,template DNA amount,Mg 2 concentration,Taq DNA polymerase amount,dNTPs concentration,random primers concentration,and PCR reaction files were studied by using PE2400.The best protocol optimum of RAPD used in the testing of vegetable seed purity was decided. |
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| Keywords: | RAPD vegetable seed purity |
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