| 运动疲劳大鼠心肌细胞L-型钙通道生物物理特性的研究 |
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| 引用本文： | 姜山,韩勇,费明旋,张守伟.运动疲劳大鼠心肌细胞L-型钙通道生物物理特性的研究[J].体育科学,2011,31(11). |
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| 作者姓名： | 姜山 韩勇 费明旋 张守伟 |
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| 作者单位： | 东北师范大学,吉林 长春,130024 |
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| 摘 要： | 目的:研究运动疲劳对大鼠心肌细胞L-型钙通道电流及生物物理学特性的影响,证明L-型钙通道直接参与到运动疲劳导致的细胞内钙浓度变化中.方法:48只大鼠雌雄不拘,随机分成对照组和运动疲劳组.采用连续15天游泳耐力训练方法建立运动疲劳大鼠模型；应用膜片钳技术检测运动疲劳对大鼠心肌细胞L-型钙通道电流的影响.结果:1)运动疲劳大鼠全细胞L-型钙电流峰值( IpeakCaL)显著增加:全细胞IpeakCaL电流从-470±11.73 pA变化到-819.90±10.78pA,n=8,P＜0.05,运动性疲劳组较对照组增幅74.60％;2)膜片钳技术检测L-型钙电流的激活和失活通道动力学特性:运动疲劳模型组大鼠心肌细胞的L-型钙电流的失活曲线右移,而激活曲线与对照组相比没有变化.结论:运动疲劳引起大鼠L-型钙通道特性改变,使得钙电流增强,导致心肌细胞内钙平衡调节紊乱.
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| 关 键 词： | L-型钙通道 激活失活曲线 运动性疲劳 心室肌细胞 鼠 动物实验 |
Study of Biophysical Properties of L-type Calcium Channel in Rat Myocardial Cells with Exercise-induced Fatigue |
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| JIANG Shan,HAN Yong,FEI Ming-xuan,ZHANG Shou-wei.Study of Biophysical Properties of L-type Calcium Channel in Rat Myocardial Cells with Exercise-induced Fatigue[J].China Sport Science,2011,31(11). |
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| Authors: | JIANG Shan HAN Yong FEI Ming-xuan ZHANG Shou-wei |
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| Institution: | JIANG Shan,HAN Yong,FEI Ming-xuan,ZHANG Shou-weiNortheast Normal University,Changchun 130024,China. |
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| Abstract: | Objective:To study the effects of exercise-induced fatigue on myocardial cells L-type calcium currents and biophysical properties in rats;to prove that L-type Ca2+ channel participates directly in the change of i.due to exercise-induced fatigue.Methods:48 rats,male or female,were divided into control group and exercise-induced fatigue group randomly.15 days endurance swimming training was adopted to establish exercise-induced fatigue models;patch clamp technique was applied to examine the effects of exercis... |
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| Keywords: | L-type calcium channel activation and inactivation curve exercise-induced fatigue myocardial cell rat |
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