cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus |
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| Authors: | Heng-mu Zhang Jian-ping Chen Qing-zhong Xue Juan-li Lei |
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| Institution: | 1.College of Agriculture and Biotechnology,Zhejiang University,Hangzhou,China;2.Virology Laboratory,Zhejiang Academy of Agricultural Sciences,Hangzhou,China |
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| Abstract: | Genome segments S8 of two Chinese isolates of rice black-streaked dwarf virus (RBSDV), one from Zhejiang Province and another from Hebei Province, were amplified by RT-PCR and sequenced. Both segments consisted of 1936 nts in full length (EMBL accession numbers were AJ297431 and AJ297432, respectively) and contained only one big open reading frame which encoded a polypeptide with molecular weight of 68kD. The two Chinese isolates shared 94.0% and 96.5% identity at nucleotide and amino acid level, respectively. They shared 94.5-94.9% and 92.5-92.9% homology with S8 of RBSDV Japanese isolate at nucleotide and amino acid level, respectively; shared 85.1-87.6% and 91.7-91.9% homology with S7 of Italian MRDV (maize rough dwarf virus). |
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| Keywords: | Rice black-streaked dwarf virus(RBSDV) Genome segment S8 Sequence analysis Biotechnology |
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