| 低温几丁质酶生产菌的筛选鉴定与产酶条件研究 |
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| 引用本文： | 王晓辉,张庆芳,迟乃玉.低温几丁质酶生产菌的筛选鉴定与产酶条件研究[J].大连大学学报,2014(3):82-87. |
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| 作者姓名： | 王晓辉 张庆芳 迟乃玉 |
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| 作者单位： | 大连大学生命科学与技术学院 |
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| 摘 要： | 以胶体几丁质为唯一碳源,从大连渤海湾的底泥样品中分离到1株高产低温几丁质酶的海洋细菌,命名为DL-06。采用单因素优化方法确定该菌株产低温几丁质酶的最佳条件,结果表明:胶体几丁质5.0 g/L,胰蛋白胨5.0 g/L,发酵温度20℃,pH 7.0,2%接种量,装液量60%,转速130 r/min,陈海水1.0 L,发酵时间30 h,在该条件下酶活力达6.87 U/mL。
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| 关 键 词： | 海洋细菌 低温几丁质酶 优化 |
Screening Identification of a Chitinolytic Bacterium Strain DL-06 and its Optimal Chitinase Fermentation Conditions |
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| WANG Xiao-hui;ZHANG Qing-fang;CHI Nai-yu.Screening Identification of a Chitinolytic Bacterium Strain DL-06 and its Optimal Chitinase Fermentation Conditions[J].Journal of Dalian University,2014(3):82-87. |
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| Authors: | WANG Xiao-hui;ZHANG Qing-fang;CHI Nai-yu |
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| Institution: | WANG Xiao-hui;ZHANG Qing-fang;CHI Nai-yu;College of Life Science and Technology,Dalian University; |
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| Abstract: | Using colloidal chitin as the sole carbon source, a marine bacteria with high productivity of cold-adapted chitinase was obtained from samples collected in Bohai bay in Dalian city of Liaoning Province, China,named as DL-06. The optimal fermentation conditions of cold-adapted chitinase-producing bacteria by single-factor experiments were determined as followed： colloidal chitin 5.0g/L, peptone 5.0 g/L, fermentation temperature 20℃, pH 7.0, inoculums dose 2%, shaking speed 130r/min, aged seawater 1.0 L and fermentation 30 h, respectively. Under these conditions, the maximum activity of cold-adapted chitinase was 6.87 U/ml. |
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| Keywords: | marine bacteria cold-adapted chitinase optimization |
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