| 外源精胺对离体铁皮石斛花芽分化过程中游离多胺含量的影响 |
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| 引用本文： | 李婷婷,唐旭,曾桢迦,颜小玉,植爽,黄作喜.外源精胺对离体铁皮石斛花芽分化过程中游离多胺含量的影响[J].内江师范学院学报,2013,28(2):36-38. |
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| 作者姓名： | 李婷婷 唐旭 曾桢迦 颜小玉 植爽 黄作喜 |
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| 作者单位： | 内江师范学院“特色农业资源研究与利用”高校重点实验室,四川 内江,641100 |
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| 基金项目： | 四川省教育厅科研项目，内江师范学院大学生科研项目 |
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| 摘 要： | 以铁皮石斛去根苗为试材,研究了外源精胺(Spm)对离体铁皮石斛花芽分化过程中内源游离多胺含量的影响.在添加14.5mg/L Spm的MS培养基上培养铁皮石斛去根苗的花芽分化率为15.0%,未添加激素的对照组的为0.在花芽分化过程中去根苗内源游离亚精胺(Spd)、Spm的含量及Spm/Put(腐胺)、Spd/Put的值均高于对照组,说明高水平的内源游离Spm、Spd含量及Spm/Put、Spd/Put有利于去根苗花芽的分化.在花芽分化过程中游离Put含量低于对照组,说明外源Spm促进了Put转化为Spd和Spm.实验揭示外源Spm可引起铁皮石斛内源游离多胺代谢及比值的变化,促进花芽分化.
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| 关 键 词： | 铁皮石斛 去根苗 花芽分化 游离多胺 |
Effects of Exogenous Spermine on Concentration of Endogenous Free Polyamines in Flower-bud Differentiation of Dendrobium officinate Kimura et Migo in Vitro |
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| LI Ting-ting
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TANG Xu
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ZENG Zhen-jia
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YAN Xiao-yu
,
ZHI Shuang
,
HUANG Zuo-xi.Effects of Exogenous Spermine on Concentration of Endogenous Free Polyamines in Flower-bud Differentiation of Dendrobium officinate Kimura et Migo in Vitro[J].Journal of Neijiang Teachers College,2013,28(2):36-38. |
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| Authors: | LI Ting-ting TANG Xu ZENG Zhen-jia YAN Xiao-yu ZHI Shuang HUANG Zuo-xi |
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| Institution: | (Key Laboratory of Colleges and Universities for Research and Utilization of Distinctive Agricultural Undertakings, Neijiang Normal University, Neijiang, Sichuan 641100, China) |
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| Abstract: | Effect of exogenous Spermine on concentration of endogenous free po]yamines in flower-bud differentiation of- Dendrobium oJ：ficinate Kimura et Migo in vitro was researched, in which derooted seedlings were collected as experimental ma terials. It was found that the rate of flower-bud differentiation in derooted seedlings cultured on MS mixed with 14.5mg/L Spm accounted for 15.0%, while the control group was 0. During the floral-bud differentiation, the endogenous concentration of free Spm, Spd and the ratio of Spm to Put, Spd to Put in derooted seedlings cultured on MS mixed with 14.5mg/L Spm excee- ded those in the control, which indicated that high levels of endogenous free Spm, Spd, Spm to Put rate and Spd to Put rate could promote the flower-bud differentiation. The concentration of free Put was lower than that in the control during the flow- er-bud differentiation process, indicating that exogenous Spermine promoted the conversion of Put to Spin and Spd. It was found that exogenous Spermine could promote the flower-bud differentiation by means of the changes of endogenous free poly amines concentration and its ratio in Dendrobium officinate Kimura et Migo. |
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| Keywords: | Dendrobium officinate Kimura et Migol derooted seedlings flower-bud differentiation free polyamines |
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