| 原生质体紫外诱变选育茶薪菇的研究 |
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| 引用本文： | 张渊,王谦,张筱梅,张焕英,张焕英.原生质体紫外诱变选育茶薪菇的研究[J].保定师专学报,2003,16(4):24-26. |
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| 作者姓名： | 张渊 王谦 张筱梅 张焕英 张焕英 |
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| 作者单位： | [1]河北大学生命科学学院，河北保定071002 [2]保定师范专科学校生物系，河北保定071051 |
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| 摘 要： | 研究了茶薪菇原生质体的形成及影响因素，并进行了以原生质体为材料的诱变育种工作．实验表明，培养5天的茶薪菇茵丝体酶解3小时原生质体数目达到最大值．原生质体经紫外线照射20秒，致死率即达70％以上．再生茵株经筛选后，原生质体再生株C16和诱变株C304产量较对照分别提高了42．7％和17．9％．
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| 关 键 词： | 茶薪菇 原生质体 诱变选育 紫外线诱变 诱变育种 |
| 文章编号： | 1008-4584(2003)04-0024-03 |
| 修稿时间： | 2003年7月6日 |
The Breeding Study by UV-inducing Protoplast of Agrocybe Chaxingu |
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| ZHANG Yuan,WANG Qian,ZHANG Xiao-mei,ZHANG Huan-ying.The Breeding Study by UV-inducing Protoplast of Agrocybe Chaxingu[J].Journal of Baoding Teachers College,2003,16(4):24-26. |
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| Authors: | ZHANG Yuan WANG Qian ZHANG Xiao-mei ZHANG Huan-ying |
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| Institution: | ZHANG Yuan1,WANG Qian1,ZHANG Xiao-mei2,ZHANG Huan-ying2 |
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| Abstract: | The breeding by UV-inducing protoplast of Agrocybe Chaxinggu and th e factors that affect protoplast proparation were studied. Under the conditions of 3 hours of enzyme hydrolysis, the burst rate of protoplast was up to max.and 20 second of UV-irradiation,the death rate was up to 70%. From the screening o f plotoplast regenerating strains, the strain C16 and C304 showed increase in yi eld 42.7%and 17.9%, compared with the original strain. |
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| Keywords: | Agrocybe Chaxingu Protoplast UV-induced mutagenesis |
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