Quantification of urinary urate with alkylamine glass-bound uricase and arylamine glass-bound peroxidase |
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| Authors: | C S Pundir A K Bhargava |
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| Institution: | (1) Biochemistry Research Laboratory, Department of Bio-Sciences, M. D. University, 124001 Rohtak, Haryana, India |
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| Abstract: | Uric acid in urine was analyzed using porcine liver uricase and horseradish peroxidase immobilized on alkylamine and arylamine
glass beads (pore diameter 55nm) respectively. The minimum detection limit was 5.0mg/0.1 ml urine. The recovery of added uric
acid was 92%. Within and between assay CVS were <1.3% and <5.3% respectively. A good correlation (r=0.93) was found between
urinary urate values obtained by a commercial kit method and the present method. The uric acid in 24hr urine of apparently
healthy adults and person sufering from various diseases was found to be 450 to 900mg/24hr, 659mg/24hr (range, mean) and 910–1400mg/24hr,
1145 mg/24hr (range, mean) respectively with the present method. |
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| Keywords: | Urate uricase peroxidase urine alkylamine glass arylamine glass |
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