Uniform yeast cell assembly via microfluidics |
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| Authors: | Chang Ya-Wen He Peng Marquez Samantha M Cheng Zhengdong |
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| Institution: | 1Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843, USA;2Maggie L. Walker Governor’s School for Government and International Studies, Richmond, Virginia 23220, USA;3Material Science and Engineering Program, Texas A&M University, College Station, Texas 77843, USA;4Professional Program in Biotechnology, Texas A&M University, College Station, Texas 77843, USA |
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| Abstract: | This paper reports the use of microfluidic approaches for the fabrication of yeastosomes (yeast-celloidosomes) based on self-assembly of yeast cells onto liquid-solid or liquid-gas interfaces. Precise control over fluidic flows in droplet- and bubble-forming microfluidic devices allows production of monodispersed, size-selected templates. The general strategy to organize and assemble living cells is to tune electrostatic attractions between the template (gel or gas core) and the cells via surface charging. Layer-by-Layer (LbL) polyelectrolyte deposition was employed to invert or enhance charges of solid surfaces. We demonstrated the ability to produce high-quality, monolayer-shelled yeastosome structures under proper conditions when sufficient electrostatic driving forces are present. The combination of microfluidic fabrication with cell self-assembly enables a versatile platform for designing synthetic hierarchy bio-structures. |
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