| 改良的二维电泳法分离水稻小穗蛋白 |
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| 引用本文： | 白昌,张素敏,刘良式.改良的二维电泳法分离水稻小穗蛋白[J].中山大学学报论丛,1989(4). |
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| 作者姓名： | 白昌 张素敏 刘良式 |
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| 摘 要： | 介绍一种适于植物材料的二维电泳方法,并对其中各有关步骤进行了讨论。该方法特別适于含干扰等电聚焦,及含使背景显色杂质的植物材料的全蛋白分离,并可获得较高分辨率及稳定的二维电泳图谱。文中还利用这种二维电泳技术,比较了籼型广亲和水稻、籼稻,粳型广亲和水稻、粳稻小穗的蛋白质差异。
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| 关 键 词： | 二维电泳 水稻 小穗蛋白质 |
Seperation of Spikelet Proteins of Oryza sativa by A Modified 2D-PAGE Method |
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| Bai Chang Zhang Sumin Liu Liangshi.Seperation of Spikelet Proteins of Oryza sativa by A Modified 2D-PAGE Method[J].Supplement to the Journal of Sun Yatsen University,1989(4). |
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| Authors: | Bai Chang Zhang Sumin Liu Liangshi |
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| Institution: | Bai Chang Zhang Sumin Liu Liangshi |
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| Abstract: | A modified two dimensional gel electrophoresis method is presented here. The procedure, which provides an excellent resolution and good reproducibility, is especially useful for the seperation of total proteins from plant tissues which contain substances interfering with the isoelectric focusing of proteins and yielding background staining. As for application, this method was used to study the differences of the total proteins between varieties of O.sativa subsp, japonica with and without wide compatibility. The differences between varieties of O.sativa subsp, indica with and without wide compatibility were also studied. |
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| Keywords: | 2D-PAGE rice proteins of rice spikelet |
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