| 药用植物虎杖毛状根DNA提取方法研究 |
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| 引用本文： | 苗晓燕,贾晓梅.药用植物虎杖毛状根DNA提取方法研究[J].保定师专学报,2009(4):67-69. |
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| 作者姓名： | 苗晓燕 贾晓梅 |
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| 作者单位： | 保定学院生物系; |
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| 摘 要： | 采用高盐低pH法、改良SDS法、CTAB法、改良CTAB法提取发根农杆菌ATCC11325感染蓼科多年生药用植物虎杖（Polygonum cuspidatum Sieb．et Zucc．）叶片产生毛状根的基因组DNA，并进行了电泳分析、含量检测及特异PCR扩增．结果表明：改良CTAB法解决了虎杖富合多酚、多糖等类物质难以提取高质量DNA的问题，提取的DNA质量较好，纯度较高，能满足进一步的实验要求．
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| 关 键 词： | 虎杖 毛状根 基因组DNA |
Research on the Methods of DNA Extraction from Polygonum cuspidatum Hairy Roots |
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| MIAO Xiao-yan,JIA Xiao-mei.Research on the Methods of DNA Extraction from Polygonum cuspidatum Hairy Roots[J].Journal of Baoding Teachers College,2009(4):67-69. |
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| Authors: | MIAO Xiao-yan JIA Xiao-mei |
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| Institution: | Department of Biology;Baoding University;Baoding 071000;China |
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| Abstract: | Genomic DNA of Polygonum cuspidatum hairy roots which is infected Agrobacterium rhizogenes ATCC11325 was extracted by means of four different methods of SDS with high salt and low pH,improved SDS,routine CTAB and improved CTAB. The quality and purity of DNA obtained by these methods were compared,and quality of DNA evaluated by agarose gel electrophoresis. The result showed that DNA sample obtained by improved CTAB method showed the highest purity and quality. It is difficult to extract high quality genomic... |
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| Keywords: | Polygonum cuspidatum hairy roots genomic DNA |
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