Calcineurin activity as an indicator of oxidative stress in normal islet cells and insulinoma cells |
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| Authors: | T K Sankaranarayanan C Subramanyam |
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| Institution: | (1) Jonaki/BRIT, CCMB Campus, 500 007 Hyderabad;(2) Department of Biochemistry, Osmania University, 500 007 Hyderabad, India |
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| Abstract: | A comparative study was conducted to evaluate calcineurin activity in normal pancreatic beta cells and insulinoma cells in
relation to their oxidative state. In comparison to normal islets, insulinoma cells had enhanced oxidative stress as evidenced
by increased content of thiobarbituric acid reactive substances. In addition, diminished activity of calcineurin in insulinoma
cells was concomitant with decreased content of reduced glutathione and glutathione peroxidase activity signifying diminished
antioxidant status in these cells. Culturing insulinoma cells in presence of the calcineurin inhibitor cyclosporin A resulted
in further decrease of calcineurin activity with restoration of glutathione peroxidase but without restoration of reduced
glutathione levels. These results indicate that an estimate of oxidative stress in pancreatic islets and insulinoma cells
can be obtained by assaying calcineurin activity. |
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| Keywords: | Calcineurin Cyclosporin Glutathione peroxidase Insulin Insulinoma Oxidative stress |
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