Microfluidic enrichment of small proteins from complex biological mixture on nanoporous silica chip |
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| Authors: | Hu Ye Gopal Ashwini Lin Kevin Peng Yang Tasciotti Ennio Zhang Xiaojing John Ferrari Mauro |
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| Institution: | 1The Methodist Hospital Research Institute, Houston, Texas 77030, USA;2Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas 78712, USA;3The University of Texas Health Science Center at Houston, Houston, Texas 77031, USA |
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| Abstract: | The growing field of miniaturized diagnostics is hindered by a lack of pre-analysis treatments that are capable of processing small sample volumes for the detection of low concentration analytes in a high-throughput manner. This letter presents a novel, highly efficient method for the extraction of low-molecular weight (LMW) proteins from biological fluids, represented by a mixture of standard proteins, using integrated microfluidic systems. We bound a polydimethylsiloxane layer patterned with a microfluidic channel onto a well-defined nanoporous silica substrate. Using rapid, pressure-driven fractionation steps, this system utilizes the size-exclusion properties of the silica nanopores to remove high molecular weight proteins while simultaneously isolating and enriching LMW proteins present in the biological sample. The introduction of the microfluidic component offers important advantages such as high reproducibility, a simple user interface, controlled environment, the ability to process small sample volumes, and precise quantification. This solution streamlines high-throughput proteomics research on many fronts and may find broad acceptance and application in clinical diagnostics and point of care detection. |
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