Supernatant decanting on a centrifugal platform |
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| Authors: | Shih Chih-Hsin Lu Chien-Hsing Yuan Wei-Li Chiang Wei-Ling Lin Chia-Hui |
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| Institution: | 1Department of Chemical Engineering, Feng Chia University, 100 Wenhua Road, Taichung 40724, Taiwan;2Department of Obstetrics and Gynecology, Taichung Veterans General Hospital, 160 Chung-Kang Rd. Sec. 3, Taichung 40705, Taiwan; Institute of Biomedical Sciences, National Chung-Hsing University, Taichung 40227, Taiwan; and Department of Obstetrics and Gynecology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan |
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| Abstract: | This study presents a novel approach to decant supernatant on a centrifugal platform. By manipulating the centrifugally induced pressure and the elastic deformation of the plastic lids in the decanting chamber, fixed amounts of the supernatant can be decanted into the detection chamber at lower rotational speeds. The experimental results showed that decanted volume is affected by the volume of deformation and the operating parameters. Factors that influence the decanting ratio are also discussed. This approach has the advantages of simple design and low manufacturing cost; further, it has no need of surface modification. It has been applied to on-disk separation of plasma from whole blood, and the results showed good stability and repeatability. |
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