| 白茅根提取物的抑菌效果研究 |
| |
| 引用本文： | 李昌灵,张建华.白茅根提取物的抑菌效果研究[J].怀化学院学报,2012(11):34-37. |
| |
| 作者姓名： | 李昌灵 张建华 |
| |
| 作者单位： | [1]怀化学院生命科学系,湖南怀化418008 [2]怀化学院湘西药用植物与民族植物学湖南省高校重点实验搴,湖南怀化418008 [3]怀化学院民族药用植物资源研究与利用湖南省重点实验室,湖南怀化418008 |
| |
| 基金项目： | 湖南省教育厅青年项目（108082）;民族药用植物资源研究与利用湖南省重点实验室项目（zDsYsJJ2012-3）;湖南省高校创新平台开放基金项目（09K106）;怀化学院科研启动资金. |
| |
| 摘 要： | 研究白茅根提取物的抑菌效果.以水、50%乙醇、乙酸乙酯和丙酮为溶剂对白茅根进行提取,将获得提取物进行抑菌实验.使用药敏纸片法和二倍稀释法测定了其提取物对产气肠杆菌、大肠杆菌、金黄色葡萄球菌、假丝酵母及枯草芽孢杆菌5种细菌的抑菌效果.乙酸乙酯提取物对于假丝酵母,水煮提取物对于大肠杆菌,丙酮提取物对于金黄色葡萄球菌,水煮提取物枯草芽孢杆菌及其50%乙醇提取物对于产气肠杆菌的抑菌效果最好,抑菌圈直径分别为12.0、13.0、13.5、13.5和15.0mm.白茅根的各种提取物对供试菌种的最低抑菌浓度(MIC)为:白茅根水煮提取物对大肠杆菌的抑菌效果最好,其MIC值为0.125 g/mL;其水煮、50%乙醇及乙酸乙酯提取物对大肠杆菌、产气肠杆菌及假丝酵母的MIC值均为0.500 g/mL;而其丙酮提取物对金黄色葡萄球菌的MIC值为0.250 g/mL.其5种提取物对革兰氏阳性、革兰氏阴性菌和真菌均有一定抑制作用,且对大肠杆菌的抑菌效果最明显.
|
| 关 键 词： | 白茅根 抑菌作用 溶剂 提取物 药敏纸片法 二倍稀释法 |
Study on Bacteriostasis Effect of Extracts of Rhizoma imperatae |
| |
| Institution: | LI Chang- ling, ZHANG Jian - hua( 1. Department of Life Science, Huaihua University, Huaihua, Hunan 418008 ; 2. Key Laboratory of Hunan Higher Education for Hunan - Western Medicinal Plant and Ethnobotany, Huaihua, Hunan 418008; 3. Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources, H uaihua, Hunan 418008 ) |
| |
| Abstract: | Antibacterial experiments of extracts from Rhizoma imperatae were performed. The extracts were obtained from Rhizoma Imperatae in water, 50% ethanol, ethyl acetate and acetone, respectively. The antibiotic effects of the extracts to Enterobacter aerogenes , Escherichia coli , Staphylococcus aureus , Saccharomyces albicans and Bacillus subtilis were tested by drug sensitive slips method and tube double dilution method, respectively. Diameter of inhibition zone of the extract in ethyl acetate to Saccharomyces albicans was 12.0 ram, the extract in water to E. coli was 13.0 mm, the extract in acetone to Staphylococcus aureus was 13.5 ram, the extract in water to B. subtiUis was 13.5 mm and the extract in 50% ethanol to Enterococcusfaecalis was 15.0 mm. Their respective MICs of the extracts to test bacteria were as follows： effect of the extract in water on E. coli was the best of all, which its MIC was 0. 125 g/mL; MIC of the extract in acetone to Staphylococcus aureus was 0.250 g/mL and its MIC was 0.500 g/mL. Their respective bacteriostasis effects of the extracts in water, 50%, ethanol and ethyl acetate to E. coli, Enterococcus faecalis and Saccharomyces albicans were all marked. The extracts to G ＋ and G - bacteria and fungus were obvious, especially to E. coll. |
| |
| Keywords: | Rhizoma imperatae bacteriostasis extracts drug sensitive slips method tube double dilution method |
| 本文献已被 维普 等数据库收录！ |
|
