| HPLC 法测定长白山牛皮杜鹃中绿原酸的含 量简 |
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| 引用本文： | 何晓燕,乔淑芬,苗婷婷.HPLC 法测定长白山牛皮杜鹃中绿原酸的含 量简[J].通化师范学院学报,2014(2):9-10. |
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| 作者姓名： | 何晓燕 乔淑芬 苗婷婷 |
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| 作者单位： | 通化师范学院生命科学学院,吉林通化134002 |
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| 基金项目： | 吉林省教育厅”十二五”科学技术研究项目（吉教科合字[2012]第360号）. |
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| 摘 要： | 采用色谱柱为Aglient ZORBAX SB-C18（250mm×4．σmm，5μm）．流动相为乙腈-0．4％瞵酸溶液（15：85），检测波长327nm，流速为1．0ml·min^-1，柱温为25℃的色谱条件，建立一种测定长由山牛皮杜鹃中绿原酸含量的HPLC方法．结果表明：绿原酸在0．513～10．26μg范围内与峰面积呈良好线性关系，平均回收率为98．9％．该测定方法，简便快捷、结果准确、重现性好．
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| 关 键 词： | 牛皮杜鹃 绿原酸 HPLC |
Determination of Chlorogenic Acid in Rhododendron chrysanthum Pall. by HPLC |
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| Institution: | HE Xiao - yan, QIAO Shu - fen, MIAO Ting - ting ( College of Life Sciences, Tonghua Normal University, Tonghua ,Jilin 134002, China) |
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| Abstract: | The chlorogenic acid in Rhododendron chrysanthum Pall. was determined with HPLC. The chlorogenic acid was separated on Aglient ZORBAX SB - C18 column （ 250mm ×4.6mm, 5/.tm）, the mobile phase was acetonitrile - 0.4% phosphoric acid solution （ 15 ： 85 ）, and the detection wave length was 327 nm, the volume flow was 1.0ml · min^-1, the volume temperature was 25 ℃. The result showed that the calibration curve was linear in the range of 0. 513 -10.26μg. |
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| Keywords: | Rhododendron chrysanthum Pall chlorogenic acid HPLC |
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