| 蛋白质谱型分析在耐甲氧西林金黄色葡萄球菌鉴定中的应用研究 |
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| 引用本文： | 张玉妥,张艳芳,刘士先,邱景富,季建军,张印则.蛋白质谱型分析在耐甲氧西林金黄色葡萄球菌鉴定中的应用研究[J].河北北方学院学报(医学版),2003,20(5):6-8. |
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| 作者姓名： | 张玉妥 张艳芳 刘士先 邱景富 季建军 张印则 |
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| 作者单位： | 1. 张家口医学院微生物学教研室,075000
2. 张家口市中心血站,075000 |
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| 摘 要： | 目的 :从生化途径探索耐甲氧西林金黄色葡萄球菌 (MRSA)鉴定的新方法及其对甲氧西林的耐药机理。方法 :采用细菌透析袋表面培养法 ,通过十二烷基硫酸钠 -聚丙烯酰胺凝胶电泳 (SDS-QAGE) ,分析菌株蛋白质代谢产物谱形。结果 :MRSA和甲氧西林敏感金黄色葡萄球菌 (MSSA)的蛋白质代谢产物SDS-PAGE谱形分析存在明显可资鉴别的差异。结论 :该方法为MRSA鉴定新方法的建立提供了可行性依据 ,为进一步研究MRSA的耐药机理提供一个可参考的研究线索
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| 关 键 词： | 耐甲氧西林金黄色葡萄球菌 聚丙烯酰胺凝胶电泳 |
| 文章编号： | 1005-8796(2003)05-06-03 |
Detection of Methicillin- Resistant Staphylococcus Aureuswith Protein Spectrum |
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| Zhang Yutuo,Zhang yinze,Zhang Yanfang,et al Zhangjiakou Medical College,Zhangjiakou ,China.Detection of Methicillin- Resistant Staphylococcus Aureuswith Protein Spectrum[J].Journal of Hebei North University:Medical Edition,2003,20(5):6-8. |
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| Authors: | Zhang Yutuo Zhang yinze Zhang Yanfang Zhangjiakou Medical College Zhangjiakou China |
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| Institution: | Zhang Yutuo,Zhang yinze,Zhang Yanfang,et al Zhangjiakou Medical College,Zhangjiakou 075000,China |
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| Abstract: | Objective: To study a new biochemical method to detect MRSA (Methi cillin-Resistant Staphylococcus Aureus)and explore mechanisms of drug-resistance in MRSA. Methods: A comparison was made between the MRSA and MSSA (Methicill in-Sensitive Staphylococcus Aureus, MSSA) in the bacteria-secreted protein and whole-cellular protein with SDS-PAGE(Sodium Dodecyl Sulfate-Polyacrylamid Gel E lectrophoresis) and the method of bacteria culture on the surface of dialytic-b ags. Results: There were significant differences in SDS-PAGE spectrum of bacter ia-secreted protein between MRSA and MSSA. Conclusions: The me thod was very usef ul to provide a new way to detect of MRSA, study mechanism of drug-resistance in MRSA, identify bacteria and investigate in epidemiology. It worthes practicing in clinic after further studying. |
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| Keywords: | Methicillin resistance staphylococcus aureus SDS-PAGE |
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