| 银杏叶总黄酮对人肝癌HepG2细胞增殖与凋亡的影响 |
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| 引用本文： | 杨利丽,耿秀芳,刘红英,潘智芳,刘顺梅.银杏叶总黄酮对人肝癌HepG2细胞增殖与凋亡的影响[J].潍坊教育学院学报,2009(2):108-110. |
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| 作者姓名： | 杨利丽 耿秀芳 刘红英 潘智芳 刘顺梅 |
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| 作者单位： | [1]潍坊医学院遗传学教研室,山东潍坊261053 [2]潍坊医学院生物化学教研室,山东潍坊261053 [3]潍坊医学院细胞生物学教研室,山东潍坊261053 |
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| 基金项目： | 致谢：对梁淑娟教授及姜大磊医师对本文所作出的贡献表示衷心的感谢! |
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| 摘 要： | 目的探讨银杏叶总黄酮对体外培养的人肝癌细胞HepG2增殖与凋亡的影响。方法将银杏叶总黄酮作用于体外培养的人肝癌细胞HepG2，MTT法检测其对HepG2细胞增殖的影响，缺口末端核苷标记（TUNNEL）法检测其对HepG2细胞凋亡的影响。结果银杏叶总黄酮对体外培养的人肝癌细胞HepG2的增殖效率下降，使凋亡细胞数增加（P〈0．01），且呈剂量依赖效应。结论银杏叶总黄酮对体外培养的人HepG2细胞增殖有抑制作用，并能诱导细胞凋亡。
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| 关 键 词： | 银杏叶总黄酮 肝肿瘤 凋亡 细胞增殖 MTT TUNEL |
The Effect of Ginkgo Biloba Leaf Flavonoids and Quercetin on Proliferation and Apoptosis of Human Liver Cancer Cell Line HepG2 |
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| YANG Li-li,GENG Xiu-fang,LIU Hong-ying,PAN Zhi-fang,LIU Shun-mei.The Effect of Ginkgo Biloba Leaf Flavonoids and Quercetin on Proliferation and Apoptosis of Human Liver Cancer Cell Line HepG2[J].Journal of Weifang Educational College,2009(2):108-110. |
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| Authors: | YANG Li-li GENG Xiu-fang LIU Hong-ying PAN Zhi-fang LIU Shun-mei |
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| Institution: | YANG Li-li, GENG Xiu-fang, LIU Hong-ying,PAN Zhi-fang, LIU Shun-mei(1.The Genetics Department of Weifang Medical College, Weifang 261053, China; 2 The Biochemistry Department of Weifang Medical College ; 3 The Cell Biology Department of Weifang Medical College ) |
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| Abstract: | Objective To investigate the effect of Ginkgo biloba leaf flavonoids on proliferation and apoptosis of human liver cancer cell line HepG2 in vitro. Methods Human liver cancer cells line,HepG2 cultured in vitro,were treated with different concentrations of the flavonoids,and then the proliferation and apoptosis of the cells was detected by MTT and TUNNEL assay. Results MTT assay showed that Ginkgo biloba leaf flavonoids inhibited the proliferation of HepG2 cells line in a dose-dependent manner（ P 〈0.01）. And the apoptotic rate of the cells was increased （ P 〈 0.01 ） . Conclusion Ginkgo biloba leaf flavonoids could inhibit the cell proliferation and promote apoptosis of human hepatoma cell line HepG2. |
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| Keywords: | Ginkgo biloba leaf flavonoids Liver caneer Apoptosis Proliferation MTT TUNEL |
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