| 黑曲霉Aspergillus nigerS菌株所产β-葡糖苷酶 的纯化和酶学性质 |
| |
| 引用本文： | 王云,曾沃坦.黑曲霉Aspergillus nigerS菌株所产β-葡糖苷酶 的纯化和酶学性质[J].中国科学院研究生院学报,2009,26(2):274-279. |
| |
| 作者姓名： | 王云 曾沃坦 |
| |
| 作者单位： | 中国科学院研究生院,北京,100049 |
| |
| 摘 要： | 利用黑曲霉Aspergillus niger S菌株, 通过液体发酵的方法, 得到了一种可同时高效水解大豆异黄酮糖苷成大豆异黄酮,水解白藜芦醇苷成白藜芦醇的糖基水解酶.该酶经硫酸铵分级沉淀、离子交换层析及分子排阻色谱纯化得单一条带, 经SDS聚丙烯酰胺凝胶电泳测得该酶的表观分子量约为165ku 1u=1.660540×10-27kg。 ,比通常所报道的传统β-葡糖苷酶都大,该酶的最适反应温度为50℃; 最适pH值为5~10.
|
| 关 键 词： | 大豆异黄酮 白藜芦醇 葡糖苷酶 纯化 性质 |
| 收稿时间： | 2008-07-25 |
| 修稿时间： | 2008-10-23 |
Purification and characterization of β-glucosidase from Aspergillus niger |
| |
| WANG Yun,ZENG Wo-Tan.Purification and characterization of β-glucosidase from Aspergillus niger[J].Journal of the Graduate School of the Chinese Academy of Sciences,2009,26(2):274-279. |
| |
| Authors: | WANG Yun ZENG Wo-Tan |
| |
| Institution: | Graduate University of the Chinese Academy of Sciences, Beijing 100049, China |
| |
| Abstract: | A high activity glucosidase, which can hydrolyze glycosides, was isolated from Aspergillus niger S strain. The enzyme was purified to one spot after ammonium sulfate precipitation, ion exchange &; size exclusion chromatography and its molecular weight was about 165ku, which was quite differ from other reported glucosidase. The optimum temperature of this enzyme was 50℃ &; the optimum pH was pH 5~10. Based on the HPLC result, this enzyme can transform glycone to aglycone of isoflavones &; resveratrol with high specific activity. |
| |
| Keywords: | soybean isoflavones resveratrol glucosidase purification characterization |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《中国科学院研究生院学报》浏览原始摘要信息 |
| 点击此处可从《中国科学院研究生院学报》下载免费的PDF全文 |
|
