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| 香菇中麦角甾醇生成维生素D_2的光化反应实验设计 | |
| 引用本文： | 白晨,张翼,黄铮宇,李晓萍,江培翃,陆红.香菇中麦角甾醇生成维生素D_2的光化反应实验设计[J].实验室研究与探索,2010,29(2). |
| 作者姓名： | 白晨 张翼 黄铮宇 李晓萍 江培翃 陆红 |
| 作者单位： | 1. 上海商学院食品学院,上海,200235 2. 复旦大学,生物科学教学实验中心 3. 复旦大学,计算机学院,上海,200433 |
| 摘 要： | 蘑菇中的麦角甾固醇经紫外线照射后可产生维生素D2,能被人体吸收利用,是天然维生素D的重要来源之一。为了增进本科生对脂溶性维生素代谢的认识和理解,设计了从香菇中提取麦角甾醇,再经光化反应将麦角甾醇转化为维生素D2的生物化学教学实验。为简化实验步骤、方便教学,试验筛选出了样品预处理、光反应及热反应的最佳条件,确定了不经皂化处理,提取物先后用254、365 nm的紫外光照射1 h后,置于85℃水浴下处理15 min实验流程。经高效液相色谱分析,测得产物收率为4.5%。 |
| 关 键 词： | 麦角甾醇 雏生素D2 光化反应 |
A Teaching Experiment on Photolysis Transformation from Ergosterol to Vitamin D_2 |
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| BAI Chen,ZHANG yi,HUANG Zhang-yu,LI Xiao-ping,JIANG Pei,LU Hong.A Teaching Experiment on Photolysis Transformation from Ergosterol to Vitamin D_2[J].Laboratory Research and Exploration,2010,29(2). | |
| Authors: | BAI Chen ZHANG yi HUANG Zhang-yu LI Xiao-ping JIANG Pei LU Hong |
| Institution: | BAI Chen 1,ZHANG Yi 2a,HUANG Zhang-yu 2b,LI Xiao-ping 2a,JIANG Pei-hong 2a,LU Hong 2a(1.Department of Food Science,Shanghai Business School,Shanghai 200235,China,a.Teaching Center of Biology,b.School of Information Science , Engineering,Fudan Univerisity,Shanghai 200433,China) |
| Abstract: | Vitamin D_2 made from ergosterol in mushrooms when it reacts with ultraviolet light,can be absorbed and utilized by the human body and is one of natural sources of vitamin D.In order to equip undergraduates with a solid understanding of metabolism of fat-soluble vitamins,a teaching experiment of biochemistry was designed where ergosterol was extracted from shiitake mushrooms,and then was transformed to vitamin D_2 by photolysis and thermal rearrangement.Optimized experimental procedure was established,which... |
| Keywords: | ergosterol vitamin D_2 photolysis reaction |
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