| 运动对RBP4诱导的胰岛素抵抗鼠骨骼肌PTP1B表达的影响 |
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| 引用本文： | 张明军.运动对RBP4诱导的胰岛素抵抗鼠骨骼肌PTP1B表达的影响[J].西安体育学院学报,2012,29(4):486-491. |
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| 作者姓名： | 张明军 |
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| 作者单位： | 惠州学院体育系,广东惠州,516007 |
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| 基金项目： | 福建省自然科学基金项目(2011J01151);莆田市科技项目(2011G04) |
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| 摘 要： | 目的研究运动对RBP4诱导的胰岛素抵抗大鼠骨骼肌PTP1B表达的影响,探讨运动影响RBP4诱导的胰岛素抵抗机制。方法 8周龄雄性SD大鼠给予重组RBP4腹腔注射,建立胰岛素抵抗鼠模型。设RBP4+运动组(RE)、RBP4+安静组(RQ)和正常安静对照组(C),RE组予3周游泳训练。ELISA、液闪计、免疫组化法、免疫印记法和RT-PCR法分别检测血清RBP4,胰岛素敏感指数QUICK I、葡萄糖摄取率和骨骼肌PTP1B、GLUT4 mRNA表达。结果 RE和RQ组血清RBP4和PTP1B表达显著高于C组,葡萄糖摄取率、QUICK I和GLUT4 mRNA表达显著低于C组;3周游泳训练后,RE组血清RBP4和骨骼肌PTP1B表达显著低于RQ组,葡萄糖摄取率、QUICK I和GLUT4 mRNA表达显著高于RQ组。结论 3周游泳运动能够显著降低RBP4诱导的胰岛素抵抗大鼠骨骼肌PTP1B表达,增加GLUT4 mRNA表达,促进葡萄糖摄取,改善胰岛素抵抗。
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| 关 键 词： | 运动 RBP4 胰岛素抵抗 PTP1B GLUT4 mRNA |
Effects of Exercise on PTP1B Expression of Skeletal Muscle in RBP4-induced Insulin Resistance Rats |
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| ZHANG Ming-jun.Effects of Exercise on PTP1B Expression of Skeletal Muscle in RBP4-induced Insulin Resistance Rats[J].Journal of Xi'an Institute of Physical Education,2012,29(4):486-491. |
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| Authors: | ZHANG Ming-jun |
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| Institution: | ZHANG Ming-jun(Department of Physical Education,Huizhou University,Huizhou 516007,China) |
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| Abstract: | Objective To study the effects of swimming on PTP1B expression of skeletal muscle in RBP4-induced insulin resistance rats and to explore mechanism of exercise on RBP4-induced insulin resistance.Methods 8-week-old SD male rats model established by intraperitoneal injection with Recombination RBP4.Model rats were divided into RBP4+swimming group(RE)and RBP4+quiet group(RQ).Normal control group(C) established too.RE group rata swam three weeks without load.ELISA,fluid flash counter,IHC,WB and RT-PCR detected serum RBP4,QUICK I,glucose uptake rate,PTP1B and GLUT4mRNA expression,respectively.Results Serum RBP4,PTP1B expression of skeletal muscle increased more in groups RE and groups RQ than group C respectively.Insulin-stimulated glucose uptake rate,QUICK I and GLUT4mRNA expression decreased more in groups RE and groups RQ than group C,respectively.Serum RBP4,and PTP1B expression of skeletal muscle decreased more in RE group than RQ group,respectively.Insulin-stimulated glucose uptake rate,QUICK I and GLUT4mRNA expression increased more in RE group than RQ group,respectively.Conclusions 3-weeks swimming could decrease PTP1B expression of skeletal muscle in RBP4-induced insulin resistance rats,increase GLUT4mRNA expression,promote glucose uptake rate and improve insulin resistance. |
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| Keywords: | swimming RBP4 insulin resistance PTP1B GLUT4 mRNA |
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