| 绵羊血浆中阿维菌素的高效液相色谱法测定 |
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| 引用本文： | 曹红,陈坚,刘红.绵羊血浆中阿维菌素的高效液相色谱法测定[J].伊犁教育学院学报,2003,16(3):120-122. |
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| 作者姓名： | 曹红 陈坚 刘红 |
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| 作者单位： | 1. 石河子大学,师范学院,新疆,石河子,832000
2. 新疆医科大学药学院,新疆,乌鲁木齐,830054 |
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| 摘 要： | 测定绵羊血浆中阿维菌素的高效液相色谱法,在样本经乙酸乙酯提取分离后,直接用高效 液相色谱法进行测定(UV245nm),所测成分为阿维菌素B1a,流动相为乙腈:0.1％磷酸- 磷酸二氢钾缓冲液=65:35;检测波长:245nm;流速1.0ml.min~(-1)。
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| 关 键 词： | 阿维菌素 绵羊血浆 乙酸乙酯 高效液相色谱 |
| 文章编号： | 1009-0487(2003)03-0120-03 |
| 修稿时间： | 2002年12月3日 |
DETERMINATION OF AVERMECTIN B1a IN SHEEP PLASMA BY HIGH- PERFORMANCE LIQUID CHROMATOGRAPHY WITH UV DETECTION |
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| Cao Hong &Chen Jian & LiuHong.DETERMINATION OF AVERMECTIN B1a IN SHEEP PLASMA BY HIGH- PERFORMANCE LIQUID CHROMATOGRAPHY WITH UV DETECTION[J].Journal of Yili Education College,2003,16(3):120-122. |
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| Authors: | Cao Hong &Chen Jian & LiuHong |
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| Abstract: | A simple and sensitive method for determination of avermectin Bla in sheep plasma is described." The sample was cleaned up with Acetic Acid Ethy Ester, and monitored by high - performance liquid chromato-graphy with UV detection at 245nm. CH3CN/0.1 % H3PO4 - KH2PO4(65/35) was selected as mobile phrase with flow rate of ImL/min. |
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| Keywords: | Avermectin Bla Sheep plasma Acetic Acid Ethy Ester high- performance liquid chromato-graphy(HPLC) |
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